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JK-6
JK-6
规格:
货期:
编号:TS260477
品牌:Testobio
产品名称: JK-6
商品货号: TS260477
<strong>Cell line </strong>: JK-6
DSMZ no. : TS260477
Species : human
Cell type : multiple myeloma
Origin : established from peripheral blood of a 75-year-old male with multiple myeloma in 1992. Cells were originally IL-6 dependent but became independent during the further course of establishment. Described to carry mutations in the K- and N-RAS genes. More cytogenetic and immunological data, including HLA types are available upon request to mutz@dsmz.de
Reference(s) : 18188
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round (plasmablasts-like) cells growing in suspension; image; image
Medium : 80% RPMI 1640 + 20% FBS + 2 mM L-glutamine
Subculture : seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days
Incubation : at 37 °C with 5% CO2
Doubling time : about 48 hours
Harvest : maximal density of ca. 1.5 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Fingerprint : standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, XMLV -
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JK-6

  • 货号: TS260477
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  • 品牌 : TESTOBIO
产品名称: JK-6
商品货号: TS260477
<strong>Cell line </strong>: JK-6
DSMZ no. : TS260477
Species : human
Cell type : multiple myeloma
Origin : established from peripheral blood of a 75-year-old male with multiple myeloma in 1992. Cells were originally IL-6 dependent but became independent during the further course of establishment. Described to carry mutations in the K- and N-RAS genes. More cytogenetic and immunological data, including HLA types are available upon request to mutz@dsmz.de
Reference(s) : 18188
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round (plasmablasts-like) cells growing in suspension; image; image
Medium : 80% RPMI 1640 + 20% FBS + 2 mM L-glutamine
Subculture : seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days
Incubation : at 37 °C with 5% CO2
Doubling time : about 48 hours
Harvest : maximal density of ca. 1.5 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Fingerprint : standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, XMLV -
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