| NCIMB number | NCIMB 8250 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Acinetobacter sp. |
| Depositor designation | Baumann94 Bouvet & Grimont73 Happold & KeyVibrio01 |
| Preservation method | Lyophilised |
| Preserved as | Acinetobacter calcoaceticus |
| Other catalogue information | Different strain from NCIMB 5178. Genospecies 11 |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | Lemco agar, pH7.4, 20C, 3d: moderate growth. slight colony variation seen at 25C, 2days |
| ACDP category | 1 |
| Additional hazard information | Although presently classed as ACDP group 1, this organism is recognised as an opportunist pathogen, particularly of immunocompromised patients, and should be handled with care. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Off White |
| Colony Opacity | Translucent |
| Cellular Shape | Stout Rods |
| Cellular Size | very small |
| Cellular Arrangement | Singly/Pairs |
| Cellular Motility | Yes |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends, Some Larger Rods Present |
| Depositor Name | W.C.Evans |
| Depositor Company | University College of Wales |
| Depositor Address | Dept. of Agric. Chem. Bangor U.K. |
| Source | Sewage containing gas works effluent |
| Date of Accession | 01/11/1951 |
| Other collection IDs | ATCC11171 CIP63.46 CCUG2491 DSM590 LMG988 |
| Yeast? | False |
| K12 | No |
| References | J. Hyg. Camb. (1932) 32 p573, F.C. Happold & A. Key J.Soc.chem. Ind. Lond. (1939) 58 p55, W.C. Evans & F.C. Happold Biochem J. (1949) 45 xxix, W.H. Parr et al Biochem J. (1951) 49 p671 B.A. Kilby Biochem J. (1951) 49 x, W.C. Evans & B.S.W. Smith Nature (1951) 168 p773, W.C. Evans et al J. Bact (1952) 63 p327, S. Dagley et al J. gen. Microbiol. (1953) 8 p1, S. Dagley J. Bact (1953) 66 p620,S. Dagley & A. Rogers Biochim. Biophys. Acta (1955) 16 p418, S. Dagley & M.D. Patel Biochim. Biophys. Acta (1956) 21 p270, S. Dagley & A.R. Johnson Biochim. Biophys. Acta (1956) 21 p441, S. Dagley & J.R.L. Walker Nature (1956) 177 p1131, S. Dagley J. gen. Microbiol. (1967) 46 pp255-266, C.A. Fewson - The growth and metabolic versatility of the Gram-negative bacterium NCIB 8250(Vibrio 01) J. gen. Microbiol. (1967) 48 pp107-110, C.A. Fewson - The identity of the Gram-negative bacterium NCIB 8250 (Vibrio 01) Biochem. J. (1968) 106 p879, D.R. Farr & R.B. Cain Biochem. J. (1969) 111(4) p461, G.R. Whistance, J.F. Dillon & D.R. Threlfall Biochem. J. (1970) 117 pp119-128, G.R. Whistance, B.S. Brown & D.R. Threlfall J. gen. Microbiol. (1970) 61(3) i, C.A. Fewson, A. Barclay & R. Eason J. Bact (1970) 101 pp568-573, J.L. Johnson et al Biochem. J. (1972) 130 p211, C.H. Self & P.D.J. Weitzman J. appl. Bact. (1972) 35 p395, G.L. Jones & E.G. Carrington Biochim. Biophys. Acta (1972) 290 pp384-388, A.M. Cook & C.A. Fewson Biochem J. (1972) 130 pp927-935, A. Livingstone & C.A. Fewson Biochem J. (1972) 130 pp937-946, A. Livingstone & C.A. Fewson Biochem J. (1972) 127(3) pp77-78, A.M. Cook & C.A. Fewson J. gen. Microbiol. (1973) 74 pp139-148, G.L. Jones et al J. appl. Bact. (1973) 36 p1, D.G. Staples & J.C. Fry Biochem. Soc. Trans. (1973) 1(6) p1258, M. Jamalludin & C.A. Fewson Int. J. Syst. Bact. (1973) 23(4) p381, R. Johnson & P.H.A. Sneath SAB Tech Series no 9 Microbial Aspects of the Deterioration of Materials (1975), E.G. Beveridge Curr. Microbiol. (1985) 12 pp235-240, M.M. Barrowman & C.A. Fewson J. gen. Microbiol. (1987) 132 pp3147-3157, L.M. Fixter et al Curr. Microbiol. 1994, vol 29, no 5, pp 273-277 Mol. Microbiol. 1995, vol 18, no 1, pp 13-20 J Gen Microbiol (1962) 27, 107-119 J Bacteriol (1968) 95, 1520-1541 I.J.S.B. (1986) 36, 228-240 Rainey FA;Lang E;Stackebrandt E (1994)The phylogenetic structure of the genus Acinetobacter. Journal FEMS Microbiol Lett 124 349-354 Diversity of Alkane Hydroxylase Systems in the Environment J.B. van Beilen et al (2003) Oil & Gas Science and Technology Rev. IFP, Vol. 58 No. 4, pp. 427-440 (Unknown), J Gen Microbiol 27, 107-119, 1962 A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter), J Bacteriol 95(5), 1520-1541, 1968 Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences, Mol Cell Probes 16(1), 1-11, 2002 Diversity and antibiotic resistance of Acinetobacter spp. in water from the source to the tap, Appl Microbiol Biotechnol , 2012 Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250, J Bacteriol 179(4), 1329-1336, 1997 Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250, Mol Microbiol 18(1), 13-20, 1995 Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus, J Bacteriol 112(2), 917-931, 1972 Molecular characterization of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II of Acinetobacter calcoaceticus, Biochem J 330 ( Pt 3), 1375-1381, 1998 Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains, Environ Microbiol 1(4), 307-317, 1999 Nucleic acid homologies among oxidase-negative Moraxella species, J Bacteriol 101, 568-573, 1970 Oxidation of homogentistic acid by cell-free extracts of a vibrio, J Gen Microbiol 28, 251-256, 1962 Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products, Int J Syst Bacteriol 46(2), 506-511, 1996 Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis, Int J Syst Bacteriol 47(3), 837-841, 1997 Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization, Int J Syst Bacteriol 49 Pt 1, 87-95, 1999 RpoN (sigma54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus, J Bacteriol 176, 3493-3499, 1994 Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species., J Clin Microbiol 44(3), 827-832, 2006 Seven novel species of Acinetobacter isolated from activated sludge, Int J Syst Evol Microbiol 53(4), 953-963, 2003 Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region, J Clin Microbiol 43(4), 1632-1639, 2005 Taxonomic studies of Acinetobacter species based on the electrophoretic analysis of enzymes, J Basic Microbiol 28(6), 363-370, 1988 Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii, Int J Syst Bacteriol 36, 228-240, 1986 The growth and metabolic versatility of the gram-negative Bacterium NCIB 8250 ("Vibrio 01"), J Gen Microbiol 46(2), 255-266, 1967 The identity of the Gram-negative bacterium NCIB 8250 ("Vibrio 01"), J Gen Microbiol 48, 107-110, 1967 The metabolic availability of phenol analogues in bacterium NCIB 8250, J Appl Bacteriol 32(3), 304-311, 1969 The phylogenetic structure of the genus Acinetobacter, FEMS Microbiol Lett 124(3), 349-353, 1994 UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus, FEMS Microbiol Lett 117(2), 137-142, 1994 |
| NCIMB number | NCIMB 8250 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Acinetobacter sp. |
| Depositor designation | Baumann94 Bouvet & Grimont73 Happold & KeyVibrio01 |
| Preservation method | Lyophilised |
| Preserved as | Acinetobacter calcoaceticus |
| Other catalogue information | Different strain from NCIMB 5178. Genospecies 11 |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | Lemco agar, pH7.4, 20C, 3d: moderate growth. slight colony variation seen at 25C, 2days |
| ACDP category | 1 |
| Additional hazard information | Although presently classed as ACDP group 1, this organism is recognised as an opportunist pathogen, particularly of immunocompromised patients, and should be handled with care. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Off White |
| Colony Opacity | Translucent |
| Cellular Shape | Stout Rods |
| Cellular Size | very small |
| Cellular Arrangement | Singly/Pairs |
| Cellular Motility | Yes |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends, Some Larger Rods Present |
| Depositor Name | W.C.Evans |
| Depositor Company | University College of Wales |
| Depositor Address | Dept. of Agric. Chem. Bangor U.K. |
| Source | Sewage containing gas works effluent |
| Date of Accession | 01/11/1951 |
| Other collection IDs | ATCC11171 CIP63.46 CCUG2491 DSM590 LMG988 |
| Yeast? | False |
| K12 | No |
| References | J. Hyg. Camb. (1932) 32 p573, F.C. Happold & A. Key J.Soc.chem. Ind. Lond. (1939) 58 p55, W.C. Evans & F.C. Happold Biochem J. (1949) 45 xxix, W.H. Parr et al Biochem J. (1951) 49 p671 B.A. Kilby Biochem J. (1951) 49 x, W.C. Evans & B.S.W. Smith Nature (1951) 168 p773, W.C. Evans et al J. Bact (1952) 63 p327, S. Dagley et al J. gen. Microbiol. (1953) 8 p1, S. Dagley J. Bact (1953) 66 p620,S. Dagley & A. Rogers Biochim. Biophys. Acta (1955) 16 p418, S. Dagley & M.D. Patel Biochim. Biophys. Acta (1956) 21 p270, S. Dagley & A.R. Johnson Biochim. Biophys. Acta (1956) 21 p441, S. Dagley & J.R.L. Walker Nature (1956) 177 p1131, S. Dagley J. gen. Microbiol. (1967) 46 pp255-266, C.A. Fewson - The growth and metabolic versatility of the Gram-negative bacterium NCIB 8250(Vibrio 01) J. gen. Microbiol. (1967) 48 pp107-110, C.A. Fewson - The identity of the Gram-negative bacterium NCIB 8250 (Vibrio 01) Biochem. J. (1968) 106 p879, D.R. Farr & R.B. Cain Biochem. J. (1969) 111(4) p461, G.R. Whistance, J.F. Dillon & D.R. Threlfall Biochem. J. (1970) 117 pp119-128, G.R. Whistance, B.S. Brown & D.R. Threlfall J. gen. Microbiol. (1970) 61(3) i, C.A. Fewson, A. Barclay & R. Eason J. Bact (1970) 101 pp568-573, J.L. Johnson et al Biochem. J. (1972) 130 p211, C.H. Self & P.D.J. Weitzman J. appl. Bact. (1972) 35 p395, G.L. Jones & E.G. Carrington Biochim. Biophys. Acta (1972) 290 pp384-388, A.M. Cook & C.A. Fewson Biochem J. (1972) 130 pp927-935, A. Livingstone & C.A. Fewson Biochem J. (1972) 130 pp937-946, A. Livingstone & C.A. Fewson Biochem J. (1972) 127(3) pp77-78, A.M. Cook & C.A. Fewson J. gen. Microbiol. (1973) 74 pp139-148, G.L. Jones et al J. appl. Bact. (1973) 36 p1, D.G. Staples & J.C. Fry Biochem. Soc. Trans. (1973) 1(6) p1258, M. Jamalludin & C.A. Fewson Int. J. Syst. Bact. (1973) 23(4) p381, R. Johnson & P.H.A. Sneath SAB Tech Series no 9 Microbial Aspects of the Deterioration of Materials (1975), E.G. Beveridge Curr. Microbiol. (1985) 12 pp235-240, M.M. Barrowman & C.A. Fewson J. gen. Microbiol. (1987) 132 pp3147-3157, L.M. Fixter et al Curr. Microbiol. 1994, vol 29, no 5, pp 273-277 Mol. Microbiol. 1995, vol 18, no 1, pp 13-20 J Gen Microbiol (1962) 27, 107-119 J Bacteriol (1968) 95, 1520-1541 I.J.S.B. (1986) 36, 228-240 Rainey FA;Lang E;Stackebrandt E (1994)The phylogenetic structure of the genus Acinetobacter. Journal FEMS Microbiol Lett 124 349-354 Diversity of Alkane Hydroxylase Systems in the Environment J.B. van Beilen et al (2003) Oil & Gas Science and Technology Rev. IFP, Vol. 58 No. 4, pp. 427-440 (Unknown), J Gen Microbiol 27, 107-119, 1962 A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter), J Bacteriol 95(5), 1520-1541, 1968 Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences, Mol Cell Probes 16(1), 1-11, 2002 Diversity and antibiotic resistance of Acinetobacter spp. in water from the source to the tap, Appl Microbiol Biotechnol , 2012 Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250, J Bacteriol 179(4), 1329-1336, 1997 Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250, Mol Microbiol 18(1), 13-20, 1995 Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus, J Bacteriol 112(2), 917-931, 1972 Molecular characterization of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II of Acinetobacter calcoaceticus, Biochem J 330 ( Pt 3), 1375-1381, 1998 Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains, Environ Microbiol 1(4), 307-317, 1999 Nucleic acid homologies among oxidase-negative Moraxella species, J Bacteriol 101, 568-573, 1970 Oxidation of homogentistic acid by cell-free extracts of a vibrio, J Gen Microbiol 28, 251-256, 1962 Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products, Int J Syst Bacteriol 46(2), 506-511, 1996 Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis, Int J Syst Bacteriol 47(3), 837-841, 1997 Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization, Int J Syst Bacteriol 49 Pt 1, 87-95, 1999 RpoN (sigma54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus, J Bacteriol 176, 3493-3499, 1994 Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species., J Clin Microbiol 44(3), 827-832, 2006 Seven novel species of Acinetobacter isolated from activated sludge, Int J Syst Evol Microbiol 53(4), 953-963, 2003 Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region, J Clin Microbiol 43(4), 1632-1639, 2005 Taxonomic studies of Acinetobacter species based on the electrophoretic analysis of enzymes, J Basic Microbiol 28(6), 363-370, 1988 Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii, Int J Syst Bacteriol 36, 228-240, 1986 The growth and metabolic versatility of the gram-negative Bacterium NCIB 8250 ("Vibrio 01"), J Gen Microbiol 46(2), 255-266, 1967 The identity of the Gram-negative bacterium NCIB 8250 ("Vibrio 01"), J Gen Microbiol 48, 107-110, 1967 The metabolic availability of phenol analogues in bacterium NCIB 8250, J Appl Bacteriol 32(3), 304-311, 1969 The phylogenetic structure of the genus Acinetobacter, FEMS Microbiol Lett 124(3), 349-353, 1994 UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus, FEMS Microbiol Lett 117(2), 137-142, 1994 |