| NCIMB number | NCIMB 12093 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Paenibacillus azotofixans |
| Depositor designation | P3L-5 |
| Preservation method | Lyophilised |
| Preserved as | Bacillus azotofixans |
| Price band | A |
| Media | 090 |
| Gas regime | aerobic |
| Growth factors (and/or information) | glucose agar, 25C, 6d: good growth, soft consistency but leaving attached growth when colony picked off agar |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Motility | Yes |
| Gram stain | Gram Variable |
| Cell inclusion bodies | Spores - Eliptical |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Source | "Wheat roots, Parana State, Brazil" |
| Isolated by | L. Seldin et al |
| Date of Accession | 05/06/1985 |
| History | L.Seldin |
| Other collection IDs | ATCC35681 DSM5976 LMG9815 NCFB2833 |
| Yeast? | False |
| K12 | No |
| References | Seldin L., van Elsas J.D. and Penido E.G.C. (1984) Int. J. syst. Bact. 34 pp451-456. Bacillus azotofixans sp. nov. a nitrogen-fixing species from Brazilian soils and grass roots. Ash C. et al (1993) Antonie van Leeuwenhoek 64 pp253-260 Int. J. syst. Bact. (1995) 45(1) pp197-198 A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae, Int J Syst Bacteriol 46(4), 988-1003, 1996 Evaluation of the hypervariable region in the 16S rDNA sequence as an index for rapid species identification in the genus Paenibacillus, J Gen Appl Microbiol 48(5), 281-285, 2002 Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus, Antonie Van Leeuwenhoek 64(3-4), 253-260, 1993 Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 52, Int J Syst Bacteriol 45, 197-198, 1995 Phylogenetic heterogeneity of the genus bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences, Lett Appl Microbiol 13, 202-206, 1991 Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 51, Int J Syst Bacteriol 44, 852, 1994 Bacillus azotofixans sp. nov. a nitrogen-fixing species from Brazilian soil and grass roots, Int J Syst Bacteriol 34, 451-456, 1984 Development and testing of a DNA macroarray to assess nitrogenase (nifH) gene diversity, Appl Environ Microbiol 70(3), 1455-1465, 2004 Differential expression of nifH and anfH genes in Paenibacillus durus analysed by reverse transcriptase-PCR and denaturing gradient gel electrophoresis, Lett Appl Microbiol 46(3), 344-349, 2008 Judicial Commission of the International Committee on Systematic Bacteriology IXth International (IUMS) Congress of Bacteriology and Applied Microbiology. Minutes of the meetings, 14, 15 and 18 August 1999, Sydney, Australia, Int J Syst Evol Microbiol 50, 2239-2244, 2000 Paenibacillus Azotofixans (Seldin Et Al. 1984) Ash Et Al. 1995 Does Not Have Priority Over Paenibacillus Durum (Smith and Cato 1974) Collins Et Al. 1994: Request for an Opinion, Int J Syst Bacteriol 48, 325, 1998 Paenibacillus durus (Collins et al. 1994, formerly Clostridium durum Smith and Cato 1974) has priority over Paenibacillus azotofixans (Seldin et al. 1984). Opinion 73, Int J Syst Evol Microbiol 53(3), 931, 2003 Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food, Int J Syst Evol Microbiol 52(2), 607-616, 2002 Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans, Appl Environ Microbiol 69(6), 3658-3662, 2003 Reclassification of Paenibacillus durum (formerly Clostridium durum Smith and Cato 1974) Collins et al. 1994 as a member of the species P. azotofixans (formerly Bacillus azotofixans Seldin et al. 1984) Ash et al. 1994, Int J Syst Bacteriol 47(2), 569-572, 1997 |
| NCIMB number | NCIMB 12093 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Paenibacillus azotofixans |
| Depositor designation | P3L-5 |
| Preservation method | Lyophilised |
| Preserved as | Bacillus azotofixans |
| Price band | A |
| Media | 090 |
| Gas regime | aerobic |
| Growth factors (and/or information) | glucose agar, 25C, 6d: good growth, soft consistency but leaving attached growth when colony picked off agar |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Motility | Yes |
| Gram stain | Gram Variable |
| Cell inclusion bodies | Spores - Eliptical |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Source | "Wheat roots, Parana State, Brazil" |
| Isolated by | L. Seldin et al |
| Date of Accession | 05/06/1985 |
| History | L.Seldin |
| Other collection IDs | ATCC35681 DSM5976 LMG9815 NCFB2833 |
| Yeast? | False |
| K12 | No |
| References | Seldin L., van Elsas J.D. and Penido E.G.C. (1984) Int. J. syst. Bact. 34 pp451-456. Bacillus azotofixans sp. nov. a nitrogen-fixing species from Brazilian soils and grass roots. Ash C. et al (1993) Antonie van Leeuwenhoek 64 pp253-260 Int. J. syst. Bact. (1995) 45(1) pp197-198 A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae, Int J Syst Bacteriol 46(4), 988-1003, 1996 Evaluation of the hypervariable region in the 16S rDNA sequence as an index for rapid species identification in the genus Paenibacillus, J Gen Appl Microbiol 48(5), 281-285, 2002 Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus, Antonie Van Leeuwenhoek 64(3-4), 253-260, 1993 Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 52, Int J Syst Bacteriol 45, 197-198, 1995 Phylogenetic heterogeneity of the genus bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences, Lett Appl Microbiol 13, 202-206, 1991 Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 51, Int J Syst Bacteriol 44, 852, 1994 Bacillus azotofixans sp. nov. a nitrogen-fixing species from Brazilian soil and grass roots, Int J Syst Bacteriol 34, 451-456, 1984 Development and testing of a DNA macroarray to assess nitrogenase (nifH) gene diversity, Appl Environ Microbiol 70(3), 1455-1465, 2004 Differential expression of nifH and anfH genes in Paenibacillus durus analysed by reverse transcriptase-PCR and denaturing gradient gel electrophoresis, Lett Appl Microbiol 46(3), 344-349, 2008 Judicial Commission of the International Committee on Systematic Bacteriology IXth International (IUMS) Congress of Bacteriology and Applied Microbiology. Minutes of the meetings, 14, 15 and 18 August 1999, Sydney, Australia, Int J Syst Evol Microbiol 50, 2239-2244, 2000 Paenibacillus Azotofixans (Seldin Et Al. 1984) Ash Et Al. 1995 Does Not Have Priority Over Paenibacillus Durum (Smith and Cato 1974) Collins Et Al. 1994: Request for an Opinion, Int J Syst Bacteriol 48, 325, 1998 Paenibacillus durus (Collins et al. 1994, formerly Clostridium durum Smith and Cato 1974) has priority over Paenibacillus azotofixans (Seldin et al. 1984). Opinion 73, Int J Syst Evol Microbiol 53(3), 931, 2003 Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food, Int J Syst Evol Microbiol 52(2), 607-616, 2002 Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans, Appl Environ Microbiol 69(6), 3658-3662, 2003 Reclassification of Paenibacillus durum (formerly Clostridium durum Smith and Cato 1974) Collins et al. 1994 as a member of the species P. azotofixans (formerly Bacillus azotofixans Seldin et al. 1984) Ash et al. 1994, Int J Syst Bacteriol 47(2), 569-572, 1997 |