| NCIMB number | NCIMB 8508 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus megaterium |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 2-3d: very good growth, soft consistency, few transparent colonies - a poorly sporulating B. megaterium. |
| ACDP category | 1 |
| Colony Edge | Entire/Trace Erose |
| Colony Surface | Smooth, Shiny |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Shape | Group 1A Rods |
| Cellular Size | large |
| Cellular Motility | Yes |
| Gram stain | Gram Variable |
| Cell inclusion bodies | Spores - Ia, Terminal To Sub-Terminal |
| Cellular (Other) | Pleomorphic, Rounded Ends |
| Depositor Name | W.C. Haynes |
| Depositor Company | Northern Utilization Research and Development Division |
| Depositor Address | Peoria Illinois U.S.A. |
| Source | Soil |
| Isolated by | Western Research Laboratory |
| Date of Accession | 01/07/1955 |
| History | Western Research Laboratory |
| Other collection IDs | ATCC10778 CCM1464 CIP59.52 DSM509 DSM2894 NCIMB8578 NRRLB-938 NCDO685 |
| Yeast? | False |
| K12 | No |
| References | Garibaldi J.a. et al (1953) Ind. Engng. Chem. 45 p838. Lewis J.C. et al (1953) Agric. Fd. Chem. 1 p897 Duff R.B. et al. (1957) Nature 179 p103. 6-O-acetylglucose - a new metabolite formed by a cobalanim producing strain of B. megaterium NCIB 8508 Duff R.B. and Farmer V.C. (1958) Biochem J. 70 p515 Duff R.B. and Webley D.M. (1958) Biochem J. 70 p520 Jacobelli G. and Tabone J. (1961) Bull. soc. Chim. biol. XLIII p1197 Tabone J. et al (1963) Annls Inst. Pasteur, Paris 104 p771 Sacks L.E. et al (1964) J. Bact. 87 p952 Holdome R.S. and Foster J.W. (1967) Antonie van Leeuwenhoek 33 p365. Lethal germination of spores of B. megaterium 9885. Guerin M. et al (1970) Eur. J. Biochem. 15 pp421-427. White P.J. (1972) J. gen. Microbiol. 71 p505 Bacillus megatherium for biosynthesis of cobalamin, Ind Eng Chem 45, 838-846, 1953 Berkeley, R. C. W., Goodfellow, M. (eds.). The aerobic endospore-forming bacteria: classification and identification. Academic Press, London 1981, (journal unknown) , 1981 Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon, Biochem J 335 ( Pt 1), 159-166, 1998 Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium, Biochem J 370(2), 505-516, 2003 pH dependence and thermostability of lipases from cultures from the ARS Culture Collection, J Ind Microbiol 13, 242-248, 1994 The nutrition of Bacillus megaterium and Bacillus cereus, J Gen Microbiol 71, 505-514, 1972 |
| NCIMB number | NCIMB 8508 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus megaterium |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 2-3d: very good growth, soft consistency, few transparent colonies - a poorly sporulating B. megaterium. |
| ACDP category | 1 |
| Colony Edge | Entire/Trace Erose |
| Colony Surface | Smooth, Shiny |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Shape | Group 1A Rods |
| Cellular Size | large |
| Cellular Motility | Yes |
| Gram stain | Gram Variable |
| Cell inclusion bodies | Spores - Ia, Terminal To Sub-Terminal |
| Cellular (Other) | Pleomorphic, Rounded Ends |
| Depositor Name | W.C. Haynes |
| Depositor Company | Northern Utilization Research and Development Division |
| Depositor Address | Peoria Illinois U.S.A. |
| Source | Soil |
| Isolated by | Western Research Laboratory |
| Date of Accession | 01/07/1955 |
| History | Western Research Laboratory |
| Other collection IDs | ATCC10778 CCM1464 CIP59.52 DSM509 DSM2894 NCIMB8578 NRRLB-938 NCDO685 |
| Yeast? | False |
| K12 | No |
| References | Garibaldi J.a. et al (1953) Ind. Engng. Chem. 45 p838. Lewis J.C. et al (1953) Agric. Fd. Chem. 1 p897 Duff R.B. et al. (1957) Nature 179 p103. 6-O-acetylglucose - a new metabolite formed by a cobalanim producing strain of B. megaterium NCIB 8508 Duff R.B. and Farmer V.C. (1958) Biochem J. 70 p515 Duff R.B. and Webley D.M. (1958) Biochem J. 70 p520 Jacobelli G. and Tabone J. (1961) Bull. soc. Chim. biol. XLIII p1197 Tabone J. et al (1963) Annls Inst. Pasteur, Paris 104 p771 Sacks L.E. et al (1964) J. Bact. 87 p952 Holdome R.S. and Foster J.W. (1967) Antonie van Leeuwenhoek 33 p365. Lethal germination of spores of B. megaterium 9885. Guerin M. et al (1970) Eur. J. Biochem. 15 pp421-427. White P.J. (1972) J. gen. Microbiol. 71 p505 Bacillus megatherium for biosynthesis of cobalamin, Ind Eng Chem 45, 838-846, 1953 Berkeley, R. C. W., Goodfellow, M. (eds.). The aerobic endospore-forming bacteria: classification and identification. Academic Press, London 1981, (journal unknown) , 1981 Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon, Biochem J 335 ( Pt 1), 159-166, 1998 Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium, Biochem J 370(2), 505-516, 2003 pH dependence and thermostability of lipases from cultures from the ARS Culture Collection, J Ind Microbiol 13, 242-248, 1994 The nutrition of Bacillus megaterium and Bacillus cereus, J Gen Microbiol 71, 505-514, 1972 |