| NCIMB number | NCIMB 12520 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus megaterium |
| Depositor designation | GW1 |
| Preservation method | Unknown |
| Other catalogue information | "Mutant derived from SW1 (NCIMB 11251). Lacks N-acetyl-LL-diaminopimelate deacylase and diaminopimelate decarboxylase. Requires LL- or meso- diaminopimelate for growth; unable to convert diaminopimelate to lysine, and therefore requires L- lysine. Incorporation of radioactive diaminopimelate is a direct measure of peptidoglycan synthesis. Requires biotin for growth at 37C" |
| Price band | A |
| Media | 256 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3 + 100ug/ml DAP, 30C, 3d: good growth. 19hr - 1mm, 64hr - 6mm, matt sc, shiny conf., soft consistency |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Very Low Convex |
| Colony Colour | Cream/Yellowish |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Depositor Name | P.J.White |
| Depositor Company | University of Sheffield |
| Depositor Address | Department of Microbiology, Western Bank Sheffield U.K. S10 2TN |
| Isolated by | A. Giles |
| Date of Isolation | 03/01/1976 |
| Date of Accession | 04/09/1987 |
| Yeast? | False |
| K12 | No |
| References | Masson H.A. and Ling J.R. (1986) J. appl. Bact. 60 pp341-349. The in vitro metabolism of free and bacterially bound 2,2-diaminopimelic acid by rumen microorganisms. |
| NCIMB number | NCIMB 12520 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus megaterium |
| Depositor designation | GW1 |
| Preservation method | Unknown |
| Other catalogue information | "Mutant derived from SW1 (NCIMB 11251). Lacks N-acetyl-LL-diaminopimelate deacylase and diaminopimelate decarboxylase. Requires LL- or meso- diaminopimelate for growth; unable to convert diaminopimelate to lysine, and therefore requires L- lysine. Incorporation of radioactive diaminopimelate is a direct measure of peptidoglycan synthesis. Requires biotin for growth at 37C" |
| Price band | A |
| Media | 256 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3 + 100ug/ml DAP, 30C, 3d: good growth. 19hr - 1mm, 64hr - 6mm, matt sc, shiny conf., soft consistency |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Very Low Convex |
| Colony Colour | Cream/Yellowish |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Depositor Name | P.J.White |
| Depositor Company | University of Sheffield |
| Depositor Address | Department of Microbiology, Western Bank Sheffield U.K. S10 2TN |
| Isolated by | A. Giles |
| Date of Isolation | 03/01/1976 |
| Date of Accession | 04/09/1987 |
| Yeast? | False |
| K12 | No |
| References | Masson H.A. and Ling J.R. (1986) J. appl. Bact. 60 pp341-349. The in vitro metabolism of free and bacterially bound 2,2-diaminopimelic acid by rumen microorganisms. |