| NCIMB number | NCIMB 8923 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Geobacillus stearothermophilus |
| Depositor designation | N.R.SmithT18 Donk |
| Preservation method | Lyophilised |
| Preserved as | Bacillus stearothermophilus |
| Other catalogue information | Formerly type strain of Bacillus stearothermophilus |
| Price band | A |
| Media | 201 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM55, pH 7.0, 55C, 20hr: good growth, butyrous consistency, large and small colonies |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | White |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Arrangement | Singly/Pairs |
| Cellular Motility | No |
| Gram stain | Gram Negative |
| Cell inclusion bodies | Spores - Terminal, Sporangium Swollen |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Source | Deteriorated canned food |
| Date of Accession | 01/11/1957 |
| Other collection IDs | ATCC12980 CCM2062 CIP66.23 DSM22 IAM11062 IFO12550 JCM2501 NCA26 NCTC10339 NCDO1768 |
| Yeast? | False |
| K12 | No |
| References | Donk L. (1920) J. Bact. 5 p373 Bergeys Manual 7th Ed. p627 Baker H. et al (1960) Can. J. Microbiol. 6 p557 Smith N.R. et al (1964) J. gen. Microbiol. 34 p269 Int. Bull. Taxon. Nomen. (1965) 15(1) pp55-59 Sutow A.B. and Welker N.E. (1967) J. Bact. 93 p1454. Chemical composition of the cell walls of B. stearothermophilus. Stevens L. and Morrison M.R. (1968) Biochem. J. 108 p633. Studies on the role of polyamines associated with the ribosomes from Bacillus stearothermophilus. Stevens L. (1969) Biochem J. 113 p117. The binding of spermine to the ribosomes and ribosomol ribonucleic acid from B. stearothermophilus. Stevens L. and Pascoe G. (1972) Biochem. J. 128 p279. The location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide. Nazina T. N. et al (2001) IJSEM 51, 433 - 446 IJSEM (2004) 54:298 alk B homologs in thermophilic bacteria of the genus Geobacillus, Mol Biol 42(2), 217-226, 2008 Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus, Int J Syst Evol Microbiol 55(3), 1171-1179, 2005 Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus, Lett Appl Microbiol 46(3), 395-401, 2008 Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus, J Gen Appl Microbiol 46(1), 1-8, 2000 Cloning and biochemical characterization of DivIVA protein from Bacillus stearothermophilus, Biologia 59(3), 389-396, 2004 Cloning and characterization of flagellin genes and identification of flagellin glycosylation from thermophilic Bacillus species, Biosci Biotechnol Biochem 73(6), 1450-1452, 2009 Cloning and characterization of the arginine-specific carbamoyl-phosphate synthetase from Bacillus stearothermophilus, Eur J Biochem 249(2), 443-449, 1997 Comparative analysis of 87,000 expressed sequence tags from the fumonisin-producing fungus Fusarium verticillioides, Fungal Genet Biol 42(10), 848-861, 2005 Comparative sequence analysis of 16S-23S rRNA internal transcribed spacers of the genus Geobacillus, Biologija 54(1), 1-6, 2008 Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus, Int J Syst Evol Microbiol 61(8), 1802-1810, 2011 Enhanced Activity of Cytidine Deaminase by Gene Family Shuffling, (misc) Hanguk Misaengmul Saengmyong Konghakhoe Chi 30, 298-304, 2002 ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980, Microbiology 146 ( Pt 9), 2175-2183, 2000 Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus, Gene 329, 187-195, 2004 Notification that new names and new combinations have appeared in volume 50, part 2, of the IJSEM, Int J Syst Evol Microbiol 51(3), 795-796, 2001 Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3 end 16S rDNA and 5 end 16S-23S ITS nucleotide sequences, Int J Syst Evol Microbiol 53(3), 695-704, 2003 Phylogenetic, inter, and intraspecific sequence analysis of spo0A gene of the genus Geobacillus, Curr Microbiol 58(6), 547-553, 2009 Regulatory features of the trp operon and the crystal structure of the trp RNA-binding attenuation protein from Bacillus stearothermophilus, J Mol Biol 289(4), 1003-1016, 1999 S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli, Microbiology 146 ( Pt 2), 273-281, 2000 S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains, Microbiology 150(4), 953-965, 2004 Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species, Gene 194(1), 25-33, 1997 Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly thermoglucosidasius); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov, Int J Syst Evol Microbiol 62(7), 1470-1485, 2012 Taxonomic richness of yeasts in Japan within subtropical and cool temperate areas, PLoS One 7(11), e50784, 2012 Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th, Int J Syst Evol Microbiol 51(2), 433-446, 2001 |
| NCIMB number | NCIMB 8923 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Geobacillus stearothermophilus |
| Depositor designation | N.R.SmithT18 Donk |
| Preservation method | Lyophilised |
| Preserved as | Bacillus stearothermophilus |
| Other catalogue information | Formerly type strain of Bacillus stearothermophilus |
| Price band | A |
| Media | 201 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM55, pH 7.0, 55C, 20hr: good growth, butyrous consistency, large and small colonies |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | White |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Arrangement | Singly/Pairs |
| Cellular Motility | No |
| Gram stain | Gram Negative |
| Cell inclusion bodies | Spores - Terminal, Sporangium Swollen |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Source | Deteriorated canned food |
| Date of Accession | 01/11/1957 |
| Other collection IDs | ATCC12980 CCM2062 CIP66.23 DSM22 IAM11062 IFO12550 JCM2501 NCA26 NCTC10339 NCDO1768 |
| Yeast? | False |
| K12 | No |
| References | Donk L. (1920) J. Bact. 5 p373 Bergeys Manual 7th Ed. p627 Baker H. et al (1960) Can. J. Microbiol. 6 p557 Smith N.R. et al (1964) J. gen. Microbiol. 34 p269 Int. Bull. Taxon. Nomen. (1965) 15(1) pp55-59 Sutow A.B. and Welker N.E. (1967) J. Bact. 93 p1454. Chemical composition of the cell walls of B. stearothermophilus. Stevens L. and Morrison M.R. (1968) Biochem. J. 108 p633. Studies on the role of polyamines associated with the ribosomes from Bacillus stearothermophilus. Stevens L. (1969) Biochem J. 113 p117. The binding of spermine to the ribosomes and ribosomol ribonucleic acid from B. stearothermophilus. Stevens L. and Pascoe G. (1972) Biochem. J. 128 p279. The location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide. Nazina T. N. et al (2001) IJSEM 51, 433 - 446 IJSEM (2004) 54:298 alk B homologs in thermophilic bacteria of the genus Geobacillus, Mol Biol 42(2), 217-226, 2008 Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus, Int J Syst Evol Microbiol 55(3), 1171-1179, 2005 Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus, Lett Appl Microbiol 46(3), 395-401, 2008 Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus, J Gen Appl Microbiol 46(1), 1-8, 2000 Cloning and biochemical characterization of DivIVA protein from Bacillus stearothermophilus, Biologia 59(3), 389-396, 2004 Cloning and characterization of flagellin genes and identification of flagellin glycosylation from thermophilic Bacillus species, Biosci Biotechnol Biochem 73(6), 1450-1452, 2009 Cloning and characterization of the arginine-specific carbamoyl-phosphate synthetase from Bacillus stearothermophilus, Eur J Biochem 249(2), 443-449, 1997 Comparative analysis of 87,000 expressed sequence tags from the fumonisin-producing fungus Fusarium verticillioides, Fungal Genet Biol 42(10), 848-861, 2005 Comparative sequence analysis of 16S-23S rRNA internal transcribed spacers of the genus Geobacillus, Biologija 54(1), 1-6, 2008 Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus, Int J Syst Evol Microbiol 61(8), 1802-1810, 2011 Enhanced Activity of Cytidine Deaminase by Gene Family Shuffling, (misc) Hanguk Misaengmul Saengmyong Konghakhoe Chi 30, 298-304, 2002 ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980, Microbiology 146 ( Pt 9), 2175-2183, 2000 Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus, Gene 329, 187-195, 2004 Notification that new names and new combinations have appeared in volume 50, part 2, of the IJSEM, Int J Syst Evol Microbiol 51(3), 795-796, 2001 Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3 end 16S rDNA and 5 end 16S-23S ITS nucleotide sequences, Int J Syst Evol Microbiol 53(3), 695-704, 2003 Phylogenetic, inter, and intraspecific sequence analysis of spo0A gene of the genus Geobacillus, Curr Microbiol 58(6), 547-553, 2009 Regulatory features of the trp operon and the crystal structure of the trp RNA-binding attenuation protein from Bacillus stearothermophilus, J Mol Biol 289(4), 1003-1016, 1999 S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli, Microbiology 146 ( Pt 2), 273-281, 2000 S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains, Microbiology 150(4), 953-965, 2004 Sequencing and phylogenetic analysis of the spoIIA operon from diverse Bacillus and Paenibacillus species, Gene 194(1), 25-33, 1997 Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly thermoglucosidasius); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov, Int J Syst Evol Microbiol 62(7), 1470-1485, 2012 Taxonomic richness of yeasts in Japan within subtropical and cool temperate areas, PLoS One 7(11), e50784, 2012 Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th, Int J Syst Evol Microbiol 51(2), 433-446, 2001 |