| NCIMB number | NCIMB 8054 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus subtilis subsp. spizizenii |
| Depositor designation | N.R.Smith231 |
| Preservation method | Lyophilised |
| Preserved as | Bacillus subtilis subsp. subtilis |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 1 days |
| Growth factors (and/or information) | 2nd Colony type irreg, undulate edge, off-white, rough, wrinkled, matt, opaque, raised, 3-4mm. Lots of spores! |
| ACDP category | 1 |
| Colony Edge | Undulate/Spreading |
| Colony Surface | Shiny, Rough/Matt, Smooth |
| Colony Shape | Irregular |
| Colony Elevation | Flat |
| Colony Colour | Off White/White |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Size | large |
| Cellular Arrangement | Single, Pairs, Chains |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Eliptical |
| Cellular (Other) | 2Nd Colony Type Has More Spores Compared With Colony Type 1. |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Date of Accession | 24/02/1950 |
| History | N.R.Smith -- K.Kellerman |
| Other collection IDs | ATCC6633 BTCC7241 BUCSAV425 CCM1999 CIP52.62 DSM347 HMGBB100 HMGBB108 HNCMB100007 HNCMB100008 IAM1069 IEMBs8/58 IFO3134 IFO3513 IFO13720 IMET10880 JCM2499 NCDO1733 NCIMB8566 NCTC10400 PCI219 VKM720 WHO9 |
| GenInfo Identifier (EMBL, GenBank & DDBJ) | JX861887 |
| WDCM number | WDCM 00003 |
| Yeast? | False |
| K12 | No |
| References | Jansen E.F. and Hirschmann (1944) Archs. Biochem. 4 p297-309. Knight B.C.J.G. and Proom H.(1950) J. gen. Microbiol. 4 p508 Charney J. et al (1951) J. Bact. 62 p145 Lewis J.C. et al (1953) Agric. Food Chem. 1 p897 Grove D.C. and Randall W.A. (1955) Assay Methods of Antibiotics p34 and 109, New York: Medical Encyclopedia Inc. Michener H.D. (1955) J. Bact. 70 p192. Pack H.D. (1961) J. Bact. 82 p933. Hooke E.J. and Ball G.M. (1963) J. appl. Bact. 26 p216 Mei H.M. and Clegg L.F.L. (1962) Nature 196 p691-693. Rowley D.B. and Newcomb H.R. (1964) J. Bact. 87 p701. Radiosensitivity of dehydrogenases and transaminases in sporogenosis. Hazeu W. and Hueck H.J. (1965) Antonie van Leeuwenhoek 31 p295. Ragland T.E. (1966) J. Bact. 91 p236. Hueck H.J. (1966) Appl. Microbiol. 14 p309 Pritikin W.B. and Romig W.R. (1966) J. Bact. 92 p291. Cook T.M. et al (1966) J. Bact. 92 p1510 Henis Y., Gould J.R. and Alexander M. (1966) Appl. Microbiol. 14 p513. Weinstein M.J. et al (1967) J. Bact. 94 p789. Biological activity of the antibiotic components of the gentamicin complex. Bickhoff E.M. et al (1968) J. agric. Fd. Chem. 16 p246. Walker M.S. and Walker J.B. (1970) J. biol. Chem. 245 p6683. Matheson A. et al (1972) Can. J. Microbiol. 18(11) p1165 Harnulu B.G. and Snygg B.G. (1972) J. appl. Bact. 35 p615. Rosenthal S.L. and Matheson A. (1973) Biochim. biophys. Acta 318 p252 Trust T.J. and Coombs R.W. (1973) Can. J. Microbiol. 19 p1341. Langner H.J. and Teufel U. (1973) Fleischwirtscheft 53(8) p1125 Walker J.B. and Skorvaga M. (1973) J. biol. Chem. 248 (7) p2435. Harnulv B.G. and Snygg B.G. (1973) J. appl. Bact. 36 p677 Rosenthal S.L. and Buchanan A.M. (1974) Biochim. biophys. Acta 363 p141 Analytical Methods Committee (1977) Analyst (Lond.) 102 pp206-210. Microbiological assay of monensin in animal feeds and pre-mixes. Jinks, Guthrie and Naylor (1985) J. clin. Microbiol. 21(5) pp826-829 Nakamura, L.K. et al. IJSB (1999) 49 (3): 1211-1215 IJSEM (2003) 53:168-170 Expression and characterization of the genes encoding azoreductases from Bacillus subtilis and Geobacillus stearothermophilus., Biosci Biotechnol Biochem 70(7), 1655-1665, 2006 U, S Pharmacopeia Microbial limit tests -- Nutritional supplements -- Preparatory testing In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 2180 The tmRNA website: reductive evolution of tmRNA in plastids and other endosymbionts, Nucleic Acids Res 32(Databaseissue), D104-D108, 2004 Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA DNA hybridization in the Bacillus subtilis group, Int J Syst Evol Microbiol 57(8), 1846-1850, 2007 Design of multiplex PCR for simultaneous detection of rope-forming Bacillus strains in Iranian bread dough, J Sci Food Agric 92(13), 2652-2656, 2012 Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14698-1, 2003 Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov, Int J Syst Bacteriol 49 Pt 3, 1211-1215, 1999 A membrane protein, EzrA, regulates assembly dynamics of FtsZ by interacting with the C-terminal tail of FtsZ, Biochemistry 46(38), 11013-11022, 2007 A novel L-Amino acid ligase from Bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis, Biosci Biotechnol Biochem 74(1), 129-134, 2010 A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin, Biosci Biotechnol Biochem 73(4), 901-907, 2009 Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633, Chem Biol 17(1), 28-37, 2010 Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis, FEMS Microbiol Ecol 60(2), 341-350, 2007 European Pharmacopeia. 2.6.1. Sterility Testing. 2.6.12. and 2.6.13 Microbiological testing of non-sterile products, (journal unknown) , 51-65, 2000 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 Identification and characterization of the structural gene sbo for, and the chemical properties of, subtilosin A, a bacteriocine produced by Bacillus subtilis ATCC 6633. Poster No. K-D 10 at VAAM-Conference, Göttingen, (journal unknown) , 1999 Medical microbiology - Susceptibility testing of patogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required fordrawing a standard curve..Berlin, Germany: Deutsches Institut fur Normung; DIN DIN 58940-2 Beiblatt 2, (journal unknown) , 2002, 2002 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11133-2, 2003 Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting, Electrophoresis 32(21), 2951-2965, 2011 Relationship between physiochemical properties, aggregation and u.v. inactivation of isolated indigenous spores in water, J Appl Microbiol 98(2), 351-363, 2005 Subtilosin production by two Bacillus subtilis subspecies and variance of the sbo-alb cluster, Appl Environ Microbiol 70(4), 2349-2353, 2004 Supplemental assay method for testing for preservative interference with sterility tests.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0903.01, 1999 Supplemental assay method for testing growth-promoting qualities of brain heart infusion agar using Bacillus subtilis spores and Candida krusei as indicator organisms.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0902.01, 1999 Supplemental assay method for testing growth promoting qualities of fluid thioglycollate medium and soybean-casein digest medium using Bacillus Subtilis spores and Candida krusei as the indicator organisms.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0900.01, 1999 The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase, Proc Natl Acad Sci U S A 96(23), 13294-13299, 1999 |
| NCIMB number | NCIMB 8054 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus subtilis subsp. spizizenii |
| Depositor designation | N.R.Smith231 |
| Preservation method | Lyophilised |
| Preserved as | Bacillus subtilis subsp. subtilis |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 1 days |
| Growth factors (and/or information) | 2nd Colony type irreg, undulate edge, off-white, rough, wrinkled, matt, opaque, raised, 3-4mm. Lots of spores! |
| ACDP category | 1 |
| Colony Edge | Undulate/Spreading |
| Colony Surface | Shiny, Rough/Matt, Smooth |
| Colony Shape | Irregular |
| Colony Elevation | Flat |
| Colony Colour | Off White/White |
| Colony Opacity | Opaque |
| Cellular Shape | Rod |
| Cellular Size | large |
| Cellular Arrangement | Single, Pairs, Chains |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Eliptical |
| Cellular (Other) | 2Nd Colony Type Has More Spores Compared With Colony Type 1. |
| Depositor Company | American Type Culture Collection (ATCC) |
| Depositor Address | 12301 Parklawn Drive Rockville Maryland 20852 U.S.A. |
| Date of Accession | 24/02/1950 |
| History | N.R.Smith -- K.Kellerman |
| Other collection IDs | ATCC6633 BTCC7241 BUCSAV425 CCM1999 CIP52.62 DSM347 HMGBB100 HMGBB108 HNCMB100007 HNCMB100008 IAM1069 IEMBs8/58 IFO3134 IFO3513 IFO13720 IMET10880 JCM2499 NCDO1733 NCIMB8566 NCTC10400 PCI219 VKM720 WHO9 |
| GenInfo Identifier (EMBL, GenBank & DDBJ) | JX861887 |
| WDCM number | WDCM 00003 |
| Yeast? | False |
| K12 | No |
| References | Jansen E.F. and Hirschmann (1944) Archs. Biochem. 4 p297-309. Knight B.C.J.G. and Proom H.(1950) J. gen. Microbiol. 4 p508 Charney J. et al (1951) J. Bact. 62 p145 Lewis J.C. et al (1953) Agric. Food Chem. 1 p897 Grove D.C. and Randall W.A. (1955) Assay Methods of Antibiotics p34 and 109, New York: Medical Encyclopedia Inc. Michener H.D. (1955) J. Bact. 70 p192. Pack H.D. (1961) J. Bact. 82 p933. Hooke E.J. and Ball G.M. (1963) J. appl. Bact. 26 p216 Mei H.M. and Clegg L.F.L. (1962) Nature 196 p691-693. Rowley D.B. and Newcomb H.R. (1964) J. Bact. 87 p701. Radiosensitivity of dehydrogenases and transaminases in sporogenosis. Hazeu W. and Hueck H.J. (1965) Antonie van Leeuwenhoek 31 p295. Ragland T.E. (1966) J. Bact. 91 p236. Hueck H.J. (1966) Appl. Microbiol. 14 p309 Pritikin W.B. and Romig W.R. (1966) J. Bact. 92 p291. Cook T.M. et al (1966) J. Bact. 92 p1510 Henis Y., Gould J.R. and Alexander M. (1966) Appl. Microbiol. 14 p513. Weinstein M.J. et al (1967) J. Bact. 94 p789. Biological activity of the antibiotic components of the gentamicin complex. Bickhoff E.M. et al (1968) J. agric. Fd. Chem. 16 p246. Walker M.S. and Walker J.B. (1970) J. biol. Chem. 245 p6683. Matheson A. et al (1972) Can. J. Microbiol. 18(11) p1165 Harnulu B.G. and Snygg B.G. (1972) J. appl. Bact. 35 p615. Rosenthal S.L. and Matheson A. (1973) Biochim. biophys. Acta 318 p252 Trust T.J. and Coombs R.W. (1973) Can. J. Microbiol. 19 p1341. Langner H.J. and Teufel U. (1973) Fleischwirtscheft 53(8) p1125 Walker J.B. and Skorvaga M. (1973) J. biol. Chem. 248 (7) p2435. Harnulv B.G. and Snygg B.G. (1973) J. appl. Bact. 36 p677 Rosenthal S.L. and Buchanan A.M. (1974) Biochim. biophys. Acta 363 p141 Analytical Methods Committee (1977) Analyst (Lond.) 102 pp206-210. Microbiological assay of monensin in animal feeds and pre-mixes. Jinks, Guthrie and Naylor (1985) J. clin. Microbiol. 21(5) pp826-829 Nakamura, L.K. et al. IJSB (1999) 49 (3): 1211-1215 IJSEM (2003) 53:168-170 Expression and characterization of the genes encoding azoreductases from Bacillus subtilis and Geobacillus stearothermophilus., Biosci Biotechnol Biochem 70(7), 1655-1665, 2006 U, S Pharmacopeia Microbial limit tests -- Nutritional supplements -- Preparatory testing In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 2180 The tmRNA website: reductive evolution of tmRNA in plastids and other endosymbionts, Nucleic Acids Res 32(Databaseissue), D104-D108, 2004 Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA DNA hybridization in the Bacillus subtilis group, Int J Syst Evol Microbiol 57(8), 1846-1850, 2007 Design of multiplex PCR for simultaneous detection of rope-forming Bacillus strains in Iranian bread dough, J Sci Food Agric 92(13), 2652-2656, 2012 Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14698-1, 2003 Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov, Int J Syst Bacteriol 49 Pt 3, 1211-1215, 1999 A membrane protein, EzrA, regulates assembly dynamics of FtsZ by interacting with the C-terminal tail of FtsZ, Biochemistry 46(38), 11013-11022, 2007 A novel L-Amino acid ligase from Bacillus subtilis NBRC3134 catalyzed oligopeptide synthesis, Biosci Biotechnol Biochem 74(1), 129-134, 2010 A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin, Biosci Biotechnol Biochem 73(4), 901-907, 2009 Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633, Chem Biol 17(1), 28-37, 2010 Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis, FEMS Microbiol Ecol 60(2), 341-350, 2007 European Pharmacopeia. 2.6.1. Sterility Testing. 2.6.12. and 2.6.13 Microbiological testing of non-sterile products, (journal unknown) , 51-65, 2000 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 Identification and characterization of the structural gene sbo for, and the chemical properties of, subtilosin A, a bacteriocine produced by Bacillus subtilis ATCC 6633. Poster No. K-D 10 at VAAM-Conference, Göttingen, (journal unknown) , 1999 Medical microbiology - Susceptibility testing of patogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required fordrawing a standard curve..Berlin, Germany: Deutsches Institut fur Normung; DIN DIN 58940-2 Beiblatt 2, (journal unknown) , 2002, 2002 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11133-2, 2003 Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting, Electrophoresis 32(21), 2951-2965, 2011 Relationship between physiochemical properties, aggregation and u.v. inactivation of isolated indigenous spores in water, J Appl Microbiol 98(2), 351-363, 2005 Subtilosin production by two Bacillus subtilis subspecies and variance of the sbo-alb cluster, Appl Environ Microbiol 70(4), 2349-2353, 2004 Supplemental assay method for testing for preservative interference with sterility tests.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0903.01, 1999 Supplemental assay method for testing growth-promoting qualities of brain heart infusion agar using Bacillus subtilis spores and Candida krusei as indicator organisms.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0902.01, 1999 Supplemental assay method for testing growth promoting qualities of fluid thioglycollate medium and soybean-casein digest medium using Bacillus Subtilis spores and Candida krusei as the indicator organisms.US Department of Agriculture; USDA, (journal unknown) , Center for Veterinary Biologics STSAM0900.01, 1999 The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase, Proc Natl Acad Sci U S A 96(23), 13294-13299, 1999 |