| NCIMB number | NCIMB 8739 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus subtilis subsp. subtilis |
| Depositor designation | ICI2/4 |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 24hr: very good growth, dry consistency. The translucent colony is a variant, translucent usually breeds translucent. |
| ACDP category | 1 |
| Colony Edge | Rhizoid |
| Colony Surface | Rough |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White |
| Colony Opacity | Translucent/Opaque |
| Cellular Shape | Thick Rod |
| Cellular Size | long |
| Cellular Arrangement | Singly |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Group 1B |
| Cellular (Other) | Parallel Sides, Rounded Ends |
| Depositor Company | ICI Ltd |
| Depositor Address | Trafford Park Manchester U.K. |
| Date of Accession | 01/03/1956 |
| Other collection IDs | ATCC11774 CCM2217 CN4267 CIP1.83 DSM2109 IMET10899 NCIMB9269 NCTC8236 |
| Yeast? | False |
| K12 | No |
| References | Society for Analytical Chemistry, Analytical Methods Committee (1963) Analyst, Lond. 88 p836. Lenkart Miller L. and Ordal Z.J. (1972) Appl. Microbiol. 24(6) p878. Thomas S. and Russell A.D. (1974) J. appl. Bact. 37 p83. Studies on the mechanism of the sporicidal action of glutaraldehyde. Nakamura, L.K. et al. IJSB (1999) 49 (3): 1211-1215 (Unknown), Br Pharmacopeia , 1315, 1968 British Pharmacopoeia Commission, Biological assay of antibiotics In: British Pharmacopoeia 1993 vol 2: London (UK), British Pharmacopoeia Commission; 1993 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 European Pharmacopoeia Commission, Microbiological assay of antibiotics In: European Pharmacopoeia 3rd ed Strasbourg, France, European Pharmacopoeia Commission; 1997 Over-expression of the Saccharomyces cerevisiae exo-beta-1,3-glucanase gene together with the Bacillus subtilis endo-beta-1,3-1,4-glucanase gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in yeast, J Biotechnol 55(1), 43-53, 1997 The first International Reference Preparation of Tobramycin, J Biol Stand 10(2), 157-168, 1982 |
| NCIMB number | NCIMB 8739 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus subtilis subsp. subtilis |
| Depositor designation | ICI2/4 |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 24hr: very good growth, dry consistency. The translucent colony is a variant, translucent usually breeds translucent. |
| ACDP category | 1 |
| Colony Edge | Rhizoid |
| Colony Surface | Rough |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White |
| Colony Opacity | Translucent/Opaque |
| Cellular Shape | Thick Rod |
| Cellular Size | long |
| Cellular Arrangement | Singly |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Group 1B |
| Cellular (Other) | Parallel Sides, Rounded Ends |
| Depositor Company | ICI Ltd |
| Depositor Address | Trafford Park Manchester U.K. |
| Date of Accession | 01/03/1956 |
| Other collection IDs | ATCC11774 CCM2217 CN4267 CIP1.83 DSM2109 IMET10899 NCIMB9269 NCTC8236 |
| Yeast? | False |
| K12 | No |
| References | Society for Analytical Chemistry, Analytical Methods Committee (1963) Analyst, Lond. 88 p836. Lenkart Miller L. and Ordal Z.J. (1972) Appl. Microbiol. 24(6) p878. Thomas S. and Russell A.D. (1974) J. appl. Bact. 37 p83. Studies on the mechanism of the sporicidal action of glutaraldehyde. Nakamura, L.K. et al. IJSB (1999) 49 (3): 1211-1215 (Unknown), Br Pharmacopeia , 1315, 1968 British Pharmacopoeia Commission, Biological assay of antibiotics In: British Pharmacopoeia 1993 vol 2: London (UK), British Pharmacopoeia Commission; 1993 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 European Pharmacopoeia Commission, Microbiological assay of antibiotics In: European Pharmacopoeia 3rd ed Strasbourg, France, European Pharmacopoeia Commission; 1997 Over-expression of the Saccharomyces cerevisiae exo-beta-1,3-glucanase gene together with the Bacillus subtilis endo-beta-1,3-1,4-glucanase gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in yeast, J Biotechnol 55(1), 43-53, 1997 The first International Reference Preparation of Tobramycin, J Biol Stand 10(2), 157-168, 1982 |