| NCIMB number | NCIMB 8649 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus atrophaeus |
| Preservation method | Lyophilised |
| Preserved as | Bacillus subtilis var globigii |
| Other catalogue information | Used in the production of spore strips to monitor ethylene oxide sterilization |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 24hr: very good growth, butyrous consistency. Norris noted smaller colonies with brown papillae. Orange/white on CM55 |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White |
| Colony Opacity | Translucent |
| Cellular Shape | Thick Rod |
| Cellular Size | long |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Group 1B |
| Cellular (Other) | Parallel Sides, Rounded Ends |
| Depositor Company | National Research Council |
| Depositor Address | Division of Applied Biology Ottawa Canada |
| Source | Strongly pigmented contaminant |
| Date of Accession | 01/10/1954 |
| Other collection IDs | ATCC 51189 CIP103406 DSM2277 IAM1633 NCTC10073 NRCB-467 |
| Yeast? | False |
| K12 | No |
| References | Phillips and Kaye (1949) Am. J. Hyg. 50 p280. BS5726 (1979) Dodd A.N. and Rumbelow J.E. (1986) J. Appl. Bact. 60 p425-433. Germination of spores of B.subtilis var. niger following exposure to gaseous ethylene oxide. Cross G.L.C. and Lach V.H. (1990) J. appl. Bact. 68 pp461-469. The effects of controlled exposure to formaldehyde vapour on spores of Bacillus globigii NCTC 10073. Fritze D & Pukall R (2001) IJSEM 51 (1) 35 - 37 - Reclassifiction of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14698-1, 2003 U, S Pharmacopeia Sterilization and sterility assurance of compendial articles In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 1976 A bacterial spore test piece for the control of ethylene oxide sterilization, J Appl Bacteriol 28(3), 349-360, 1965 A SIMPLE PHENYLALANINE METHOD FOR DETECTING PHENYLKETONURIA IN LARGE POPULATIONS OF NEWBORN INFANTS, Pediatrics 32, 338-343, 1963 A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis, J Bacteriol 182(8), 2307-2310, 2000 Detection of Molecular Diversity in Bacillus atrophaeus by Amplified Fragment Length Polymorphism Analysis, Appl Environ Microbiol 70(5), 2786-2790, 2004 Formation of dry-heat resistant Bacillus subtilis var. niger spores as influenced by the composition of the sporulation medium, Antonie van Leeuwenhoek 42, 387-395, 1976 Inactivation of microorganisms by oxygen gas plasma, Curr Microbiol 18, 275-276, 1989 Kinetik-Studie über die Abtötung von Bacillus-subtilis-sporen in Ethylenoxid-Sterilisationsprozessen, Zent Steril 10, 158-167, 2002 Plastic containers for intravenous injection.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 15747, 2003 Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 3826-1, 2003 Prier, J. E. et al. (eds.). Quality control in microbiology. Univ. Park Press, Baltimore 1975, (journal unknown) , 25-39, 1975 Quality control in sterilization procedures. Biological indicators, In Quality control in microbiology (ed Prier, JE et al) , 25, 1975 Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus, Int J Syst Evol Microbiol 51(1), 35-37, 2001 Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov, Int J Syst Bacteriol 49 Pt 3, 1211-1215, 1999 Sterilization of health care products--Biological indicators--Part 2: Biological indicators for ethylene oxide sterilization.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11138-2, 1994 Sterilization of single-use medical devices incorporating materials of animal origin--Validation and routine control of sterilization by liquid chemical sterilants.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14160, 1998 Sterilizing effects of high-intensity airborn sonic and ultrasonic waves, Appl Microbiol 14, 732-736, 1996 Sterilizing effects of high-intensity airborne sonic and ultrasonic waves, Appl Microbiol 14(5), 732-736, 1966 Structure of the bacterial respiratory-chain system. Respiration of Bacillus subtilis spheroplasts as a function of the osmotic pressure of the medium, Biochim Biophys Acta 62, 145-152, 1962 Taxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov, Int J Syst Bacteriol 39, 295-300, 1989 Use of gaseous antimicrobial agents with special reference to ethylene oxide, J Appl Bacteriol 30(1), 92-100, 1967 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/16316067 |
| NCIMB number | NCIMB 8649 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Bacillus atrophaeus |
| Preservation method | Lyophilised |
| Preserved as | Bacillus subtilis var globigii |
| Other catalogue information | Used in the production of spore strips to monitor ethylene oxide sterilization |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | CM3, 30C, 24hr: very good growth, butyrous consistency. Norris noted smaller colonies with brown papillae. Orange/white on CM55 |
| ACDP category | 1 |
| Colony Edge | Entire |
| Colony Surface | Smooth |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White |
| Colony Opacity | Translucent |
| Cellular Shape | Thick Rod |
| Cellular Size | long |
| Cellular Motility | Yes |
| Gram stain | Gram Positive |
| Cell inclusion bodies | Spores - Group 1B |
| Cellular (Other) | Parallel Sides, Rounded Ends |
| Depositor Company | National Research Council |
| Depositor Address | Division of Applied Biology Ottawa Canada |
| Source | Strongly pigmented contaminant |
| Date of Accession | 01/10/1954 |
| Other collection IDs | ATCC 51189 CIP103406 DSM2277 IAM1633 NCTC10073 NRCB-467 |
| Yeast? | False |
| K12 | No |
| References | Phillips and Kaye (1949) Am. J. Hyg. 50 p280. BS5726 (1979) Dodd A.N. and Rumbelow J.E. (1986) J. Appl. Bact. 60 p425-433. Germination of spores of B.subtilis var. niger following exposure to gaseous ethylene oxide. Cross G.L.C. and Lach V.H. (1990) J. appl. Bact. 68 pp461-469. The effects of controlled exposure to formaldehyde vapour on spores of Bacillus globigii NCTC 10073. Fritze D & Pukall R (2001) IJSEM 51 (1) 35 - 37 - Reclassifiction of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14698-1, 2003 U, S Pharmacopeia Sterilization and sterility assurance of compendial articles In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 1976 A bacterial spore test piece for the control of ethylene oxide sterilization, J Appl Bacteriol 28(3), 349-360, 1965 A SIMPLE PHENYLALANINE METHOD FOR DETECTING PHENYLKETONURIA IN LARGE POPULATIONS OF NEWBORN INFANTS, Pediatrics 32, 338-343, 1963 A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis, J Bacteriol 182(8), 2307-2310, 2000 Detection of Molecular Diversity in Bacillus atrophaeus by Amplified Fragment Length Polymorphism Analysis, Appl Environ Microbiol 70(5), 2786-2790, 2004 Formation of dry-heat resistant Bacillus subtilis var. niger spores as influenced by the composition of the sporulation medium, Antonie van Leeuwenhoek 42, 387-395, 1976 Inactivation of microorganisms by oxygen gas plasma, Curr Microbiol 18, 275-276, 1989 Kinetik-Studie über die Abtötung von Bacillus-subtilis-sporen in Ethylenoxid-Sterilisationsprozessen, Zent Steril 10, 158-167, 2002 Plastic containers for intravenous injection.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 15747, 2003 Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 3826-1, 2003 Prier, J. E. et al. (eds.). Quality control in microbiology. Univ. Park Press, Baltimore 1975, (journal unknown) , 25-39, 1975 Quality control in sterilization procedures. Biological indicators, In Quality control in microbiology (ed Prier, JE et al) , 25, 1975 Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus, Int J Syst Evol Microbiol 51(1), 35-37, 2001 Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov, Int J Syst Bacteriol 49 Pt 3, 1211-1215, 1999 Sterilization of health care products--Biological indicators--Part 2: Biological indicators for ethylene oxide sterilization.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11138-2, 1994 Sterilization of single-use medical devices incorporating materials of animal origin--Validation and routine control of sterilization by liquid chemical sterilants.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14160, 1998 Sterilizing effects of high-intensity airborn sonic and ultrasonic waves, Appl Microbiol 14, 732-736, 1996 Sterilizing effects of high-intensity airborne sonic and ultrasonic waves, Appl Microbiol 14(5), 732-736, 1966 Structure of the bacterial respiratory-chain system. Respiration of Bacillus subtilis spheroplasts as a function of the osmotic pressure of the medium, Biochim Biophys Acta 62, 145-152, 1962 Taxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov, Int J Syst Bacteriol 39, 295-300, 1989 Use of gaseous antimicrobial agents with special reference to ethylene oxide, J Appl Bacteriol 30(1), 92-100, 1967 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/16316067 |