| NCIMB number | NCIMB 5920 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Cronobacter sakazakii |
| Depositor designation | 502 |
| Preservation method | Lyophilised |
| Preserved as | Enterobacter sakazakii |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 2 days |
| Growth factors (and/or information) | NA, 30C, 24hr: butyrous consistency |
| ACDP category | 2 |
| Hazard information | This species has been associated with meningitis, bacteraemia, brain abscesses, in newborn infants. |
| Additional hazard information | Ref: J J Farmer et al (1985) J. Clin. Micro. 21 (1), 46-76. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Yellow |
| Colony Opacity | Semi Opaque |
| Cellular Shape | Rod |
| Cellular Size | very short |
| Cellular Arrangement | Singly |
| Cellular Motility | Yes |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Date of Accession | 13/12/1951 |
| History | R.D.Gray |
| Other collection IDs | ATCC12868 BUCSAV144 CCM1902 NCDO713 NCTC5920 |
| Yeast? | False |
| K12 | No |
| References | Wilkinson J.F., Duguid J.P. and Edmunds P.N. (1954) J. gen. Microbiol. 11 p59 Dudman W.F. and Wilkinson J.F. (1956) Biochem. J. 62(2) pp289-295 (Farmer et al. 1980) Iversen et al. 2008 Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods, Antonie Van Leeuwenhoek 99(2), 257-269, 2011 Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter Species, J Clin Microbiol 42(11), 5368-5370, 2004 Phylogeny and prediction of genetic similarity of Cronobacter and related taxa by multilocus sequence analysis (MLSA), Int J Food Microbiol 136(2), 152-158, 2009 Real time PCR using TaqMan and SYBR Green for detection of Enterobacter sakazakii in infant formula, J Microbiol Methods 65(1), 21-31, 2006 The development of an O serotyping scheme for Cronobacter sakazakii, Appl Environ Microbiol , 2011 The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1, BMC Evol Biol 7, 64, 2007 Use of novel species-specific PCR primers targeted to DNA gyrase subunit B (gyrB) gene for species identification of the Cronobacter sakazakii and Cronobacter dublinensis, Mol Cell Probes 27(1), 15-18, 2013 |
| NCIMB number | NCIMB 5920 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Cronobacter sakazakii |
| Depositor designation | 502 |
| Preservation method | Lyophilised |
| Preserved as | Enterobacter sakazakii |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 2 days |
| Growth factors (and/or information) | NA, 30C, 24hr: butyrous consistency |
| ACDP category | 2 |
| Hazard information | This species has been associated with meningitis, bacteraemia, brain abscesses, in newborn infants. |
| Additional hazard information | Ref: J J Farmer et al (1985) J. Clin. Micro. 21 (1), 46-76. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Yellow |
| Colony Opacity | Semi Opaque |
| Cellular Shape | Rod |
| Cellular Size | very short |
| Cellular Arrangement | Singly |
| Cellular Motility | Yes |
| Gram stain | Gram Negative |
| Cellular (Other) | Straight Axis, Parallel Sides, Rounded Ends |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Date of Accession | 13/12/1951 |
| History | R.D.Gray |
| Other collection IDs | ATCC12868 BUCSAV144 CCM1902 NCDO713 NCTC5920 |
| Yeast? | False |
| K12 | No |
| References | Wilkinson J.F., Duguid J.P. and Edmunds P.N. (1954) J. gen. Microbiol. 11 p59 Dudman W.F. and Wilkinson J.F. (1956) Biochem. J. 62(2) pp289-295 (Farmer et al. 1980) Iversen et al. 2008 Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods, Antonie Van Leeuwenhoek 99(2), 257-269, 2011 Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter Species, J Clin Microbiol 42(11), 5368-5370, 2004 Phylogeny and prediction of genetic similarity of Cronobacter and related taxa by multilocus sequence analysis (MLSA), Int J Food Microbiol 136(2), 152-158, 2009 Real time PCR using TaqMan and SYBR Green for detection of Enterobacter sakazakii in infant formula, J Microbiol Methods 65(1), 21-31, 2006 The development of an O serotyping scheme for Cronobacter sakazakii, Appl Environ Microbiol , 2011 The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1, BMC Evol Biol 7, 64, 2007 Use of novel species-specific PCR primers targeted to DNA gyrase subunit B (gyrB) gene for species identification of the Cronobacter sakazakii and Cronobacter dublinensis, Mol Cell Probes 27(1), 15-18, 2013 |