| NCIMB number | NCIMB 11508 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Enterococcus faecium |
| Preservation method | Lyophilised |
| Preserved as | Streptococcus faecium |
| Other catalogue information | Grumbach serotype 11 |
| Price band | A |
| Media | 079 |
| Gas regime | aerobic |
| Incubation period | 1 days |
| Growth factors (and/or information) | Blood agar, 37C, 2d: good growth, grey with very pale orange centre, non haemolytic |
| ACDP category | 2 |
| Hazard information | This genus has been associated with nosocomial infections, especially of urinary tract. |
| Additional hazard information | Ref: R C Moellering, Clinical Infectious Diseases 1992, 14, 1173-8. Emergence of Enterococcus as a significant pathogen. |
| Colony Edge | Entire |
| Colony Surface | Shiny |
| Colony Shape | Circular |
| Colony Elevation | Raised |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Arrangement | Singly/Pairs/Cluster |
| Gram stain | Gram Positive |
| Cellular (Other) | On Glucose Agar - Oval Coccobacilli, Singly |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Isolated by | A. Grumbach |
| Date of Isolation | 01/01/1946 |
| Date of Accession | 21/05/1979 |
| History | A.Grumbach |
| Other collection IDs | ATCC19434 CIP103014 DSM20477 NCDO942 NCTC7171 |
| WDCM number | WDCM 00010 |
| Yeast? | False |
| K12 | No |
| References | Sneath and Skerman (1968) Int. J. syst. Bact. 16 p1 Schleifer K.W. and Kilpper-Balz R. (1984) Int. J. syst. Bact. 34 p31. J Appl Micro (1998) 85, 25-36 cpnDB: a chaperonin sequence database, Genome Res 14(8), 1669-1675, 2004 Division of the genus Enterococcus into species groups using PCR-based molecular typing methods, Microbiology 144 ( Pt 5), 1171-1179, 1998 Evidence for horizontal gene transfer in evolution of elongation factor Tu in enterococci, J Bacteriol 182(24), 6913-6920, 2000 Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes, Microbiology 151(7), 2141-2150, 2005 Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species, J Clin Microbiol 38(11), 3991-3993, 2000 Phylogeny and identification of Enterococci by atpA gene sequence analysis, J Clin Microbiol 43(5), 2224-2230, 2005 Identification of Enterococcus spp. based on specific hybridisation with 16S rDNA probes, J Microbiol Methods 50(2), 115-121, 2002 Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci, J Clin Microbiol 38(1), 415-418, 2000 Utilization of tmRNA sequences for bacterial identification, BMC Microbiol 1, 20, 2001 Characterization of different food-isolated Enterococcus strains by MALDI-TOF mass fingerprinting, Electrophoresis 34(15), 2240-2250, 2013 Identification of clinically relevant enterococcus species by direct sequencing of groES and spacer region, J Clin Microbiol 43(1), 235-241, 2005 Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences, J Clin Microbiol 38(11), 3953-3959, 2000 Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus: Evidence that E. thailandicus Is a Senior Subjective Synonym of "E. sanguinicola" and Confirmation of E. caccae as a Species Distinct from E. silesiacus, J Clin Microbiol 49(7), 2676-2679, 2011 Designation of the provisional new enterococcus species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004, J Clin Microbiol 46(10), 3473-3476, 2008 The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations, Food Microbiol 21(4), 481-487, 2004 Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer Region, J Clin Microbiol 42(6), 2651-2657, 2004 Characteristics and identification of enterocins produced by Enterococcus faecium JCM 5804T, J Appl Microbiol 95(2), 294-300, 2003 Cloning of insertion sequence IS1485 from Enterococcus species, Plasmid 42(1), 42-44, 1999 Development of a PCR assay for rapid detection of enterococci, J Clin Microbiol 37(11), 3497-3503, 1999 Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine, Int J Syst Bacteriol 47(2), 555-561, 1997 Presence of variations in ribosomal protein L16 corresponding to susceptibility of enterococci to oligosaccharides (Avilamycin and evernimicin), Antimicrob Agents Chemother 44(12), 3425-3427, 2000 Primary and secondary structures of rRNA spacer regions in enterococci, Microbiology 143 ( Pt 3), 823-834, 1997 rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella, J Clin Microbiol 42(2), 497-504, 2004 Use of the manganese-dependent superoxide dismutase gene sodA for rapid identification of recently described enterococcal species, Folia Microbiol (Praha) 57(5), 439-442, 2012 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/10543832 |
| NCIMB number | NCIMB 11508 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Enterococcus faecium |
| Preservation method | Lyophilised |
| Preserved as | Streptococcus faecium |
| Other catalogue information | Grumbach serotype 11 |
| Price band | A |
| Media | 079 |
| Gas regime | aerobic |
| Incubation period | 1 days |
| Growth factors (and/or information) | Blood agar, 37C, 2d: good growth, grey with very pale orange centre, non haemolytic |
| ACDP category | 2 |
| Hazard information | This genus has been associated with nosocomial infections, especially of urinary tract. |
| Additional hazard information | Ref: R C Moellering, Clinical Infectious Diseases 1992, 14, 1173-8. Emergence of Enterococcus as a significant pathogen. |
| Colony Edge | Entire |
| Colony Surface | Shiny |
| Colony Shape | Circular |
| Colony Elevation | Raised |
| Colony Colour | Off White |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Arrangement | Singly/Pairs/Cluster |
| Gram stain | Gram Positive |
| Cellular (Other) | On Glucose Agar - Oval Coccobacilli, Singly |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Isolated by | A. Grumbach |
| Date of Isolation | 01/01/1946 |
| Date of Accession | 21/05/1979 |
| History | A.Grumbach |
| Other collection IDs | ATCC19434 CIP103014 DSM20477 NCDO942 NCTC7171 |
| WDCM number | WDCM 00010 |
| Yeast? | False |
| K12 | No |
| References | Sneath and Skerman (1968) Int. J. syst. Bact. 16 p1 Schleifer K.W. and Kilpper-Balz R. (1984) Int. J. syst. Bact. 34 p31. J Appl Micro (1998) 85, 25-36 cpnDB: a chaperonin sequence database, Genome Res 14(8), 1669-1675, 2004 Division of the genus Enterococcus into species groups using PCR-based molecular typing methods, Microbiology 144 ( Pt 5), 1171-1179, 1998 Evidence for horizontal gene transfer in evolution of elongation factor Tu in enterococci, J Bacteriol 182(24), 6913-6920, 2000 Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes, Microbiology 151(7), 2141-2150, 2005 Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species, J Clin Microbiol 38(11), 3991-3993, 2000 Phylogeny and identification of Enterococci by atpA gene sequence analysis, J Clin Microbiol 43(5), 2224-2230, 2005 Identification of Enterococcus spp. based on specific hybridisation with 16S rDNA probes, J Microbiol Methods 50(2), 115-121, 2002 Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci, J Clin Microbiol 38(1), 415-418, 2000 Utilization of tmRNA sequences for bacterial identification, BMC Microbiol 1, 20, 2001 Characterization of different food-isolated Enterococcus strains by MALDI-TOF mass fingerprinting, Electrophoresis 34(15), 2240-2250, 2013 Identification of clinically relevant enterococcus species by direct sequencing of groES and spacer region, J Clin Microbiol 43(1), 235-241, 2005 Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences, J Clin Microbiol 38(11), 3953-3959, 2000 Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus: Evidence that E. thailandicus Is a Senior Subjective Synonym of "E. sanguinicola" and Confirmation of E. caccae as a Species Distinct from E. silesiacus, J Clin Microbiol 49(7), 2676-2679, 2011 Designation of the provisional new enterococcus species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004, J Clin Microbiol 46(10), 3473-3476, 2008 The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations, Food Microbiol 21(4), 481-487, 2004 Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer Region, J Clin Microbiol 42(6), 2651-2657, 2004 Characteristics and identification of enterocins produced by Enterococcus faecium JCM 5804T, J Appl Microbiol 95(2), 294-300, 2003 Cloning of insertion sequence IS1485 from Enterococcus species, Plasmid 42(1), 42-44, 1999 Development of a PCR assay for rapid detection of enterococci, J Clin Microbiol 37(11), 3497-3503, 1999 Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine, Int J Syst Bacteriol 47(2), 555-561, 1997 Presence of variations in ribosomal protein L16 corresponding to susceptibility of enterococci to oligosaccharides (Avilamycin and evernimicin), Antimicrob Agents Chemother 44(12), 3425-3427, 2000 Primary and secondary structures of rRNA spacer regions in enterococci, Microbiology 143 ( Pt 3), 823-834, 1997 rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella, J Clin Microbiol 42(2), 497-504, 2004 Use of the manganese-dependent superoxide dismutase gene sodA for rapid identification of recently described enterococcal species, Folia Microbiol (Praha) 57(5), 439-442, 2012 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/10543832 |