| NCIMB number | NCIMB 6571 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Staphylococcus aureus subsp. aureus |
| Depositor designation | 3R7089 OxfordH |
| Preservation method | Liquid nitrogen & Lyophilised |
| Preserved as | Micrococcus pyogenes var aureus |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | NA, 30C, 48hr: good growth |
| ACDP category | 2 |
| Hazard information | This species has been associated with food poisoning, furuncles, impetigo, pneumonia, mastitis, enterocolitis, urogenital infections, toxic shock syndrome, meningitis, endocarditis, toxic epidermal necrolysis and various abscesses. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White/Colourless |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | < 1 u |
| Cellular Arrangement | Singly/Pairs/Cluster |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Isolated by | J. Stokes |
| Date of Accession | 02/02/1964 |
| History | N.G.Heatley |
| Other collection IDs | ATCC9144 CCM2107 CIP53.154 CN6457 DSM683 NCTC6571 NRRLB-3141 NCDO907 |
| WDCM number | WDCM 00035 |
| Yeast? | False |
| K12 | No |
| References | Heatley N.G. (1944) Biochem J. 38 p61. A method for the assay of penicillin Cook A.M. et al (1956) J. Pharm. Pharmacol. 8 p721 Hugo W.B. and Stretton R.G. (1964) Nature 202 p1217. Action of quinacillin on Staphylococcus aureus. Silvestri L.G. and Hill L.R. (1965) J. Bact. 90 p136. Agreement between DNA base composition and taxometric classification of gram positive bacteria. Allwood M.C. and Russell R.D. (1966) Lab. Pract. 15 p1132. The storage of washed suspensions of Staphylococcus aureus. Roscoe J. and Abraham E.P. (1966) Biochem J. 99 p793. Experiments relating to the biosynthesis of bacilysin Watts F.W.C. (1967) J. med. Lab. Tech. 24 p129. Rules and Regulations "Antibiotic susceptibility discs" Federal Register 37 20525-20529 (1972) Bloomfield S. (1974) J. appl. Bact. 37 p117 Owens J.J. (1974) J. appl. Bact. 37 p137 Dastidar S.G. et al (1974) J. gen. Microbiol. 84 p245 Coyle M.B. et al (1976) Antimicrobial Agents Chemother. 10 pp436-440. Reproducibility of control strains for antibiotic susceptibility testing WHO Expert Committee on Biological Standardization 28 Report. Technical Report Series 610, Genf 1977. Requirements for antibiotic susceptibility tests. I. Agar diffusion tests using antibiotic susceptibility discs. Code of Federal Regulations title 21 part 460 1977 & 1978 National Committee on clinical laboratory standards. Performance standards for antimicrobial disc susceptibility test. Approved standard ASM-2, Villanova Ps (1979) Lenelter E.H. et al Manual of Clinical Microbiology (3rd ed.) American Society for Microbiology, Washington (1980) Sourcebook of Experiments for the Teaching of Microbiology, Eds. S.B. Primrose and A.C. Wardlaw, Academic Press 1982. [Expt. 46, 73] J. appl. Bact. (1985) 59 pp537-543 (Unknown), 34 J.Clin.Microbiol., 1995 (Unknown), 90 J.Antimicrob.Chemother., 1994 (Unknown), Analytical Microbiology J.Bacteriol., 1965, 1972 AOAC International, Chlortetracycline HCl in feeds, turbidimetric method Gaithersburg, MD, AOAC International; AOAC Official Method 977.37. U, S Pharmacopeia Antibiotics--microbial assays In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 1690 U, S Pharmacopeia Antibiotics--Microbial assays In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1996 Iodophors for use in the dairying industry.Sydney, NSW, Australia, (journal unknown) , Standards Australia AS 1398-1998 A method for the assay of penicillin, Biochem J 38(1), 61-65, 1944 Agreement Between Deoxyribonucleic Acid Base Composition and Taxometric Classification of Gram-Positive Cocci, J Bacteriol 90(1), 136-140, 1965 Beta-lactam resistance in Staphylococcus aureus cells that do not require a cell wall for integrity, Antimicrob Agents Chemother 49(12), 5075-5080, 2005 British Pharmacopoeia Commission, Biological assay of antibiotics In: British Pharmacopoeia 1993 vol 2: London (UK), British Pharmacopoeia Commission; 1993 European Pharmacopoeia Commission, Microbiological assay of antibiotics In: European Pharmacopoeia 3rd ed Strasbourg, France, European Pharmacopoeia Commission; 1997 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 Identification of a novel gene cluster encoding staphylococcal exotoxin-like proteins: characterization of the prototypic gene and its protein product, SET1, Infect Immun 68(8), 4407-4415, 2000 Kavanagh, F. (ed.). Analytical Microbiology, vol. II. Academic Press, New York 1972, (journal unknown) , 1972 Microbial assay for antibiotics.Tokyo, Japan, (journal unknown) , Japanese Pharmacopoeia Rapid screening for putative exported proteins from Staphylococcus aureus using alkaline phosphatase as a reporter molecule, Mol Biotechnol 15(1), 11-20, 2000 Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting, Electrophoresis 32(21), 2951-2965, 2011 Staphylococcus aureus subsp. anaerobius subsp. nov., the causal agent of abscess disease of sheep, Int J Syst Bacteriol 35, 99-102, 1985 Water microbiology. Method 16: Bactericidal efficiency of water disinfecting tablets.Sydney, NSW, Australia: Standards Australia, (journal unknown) , Standards Australia AS/NZS 4276.16, 1999 Water microbiology. Method 20: Examination for coagulase positive staphylococci, including Staphylococcus aureus, by memebrane filtration.Sydney, NSW, Australia: Standards Australia, (journal unknown) , Standards Australia AS/NZS 4276.20, 2003 |
| NCIMB number | NCIMB 6571 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Staphylococcus aureus subsp. aureus |
| Depositor designation | 3R7089 OxfordH |
| Preservation method | Liquid nitrogen & Lyophilised |
| Preserved as | Micrococcus pyogenes var aureus |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | NA, 30C, 48hr: good growth |
| ACDP category | 2 |
| Hazard information | This species has been associated with food poisoning, furuncles, impetigo, pneumonia, mastitis, enterocolitis, urogenital infections, toxic shock syndrome, meningitis, endocarditis, toxic epidermal necrolysis and various abscesses. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Low Convex |
| Colony Colour | White/Colourless |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | < 1 u |
| Cellular Arrangement | Singly/Pairs/Cluster |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Isolated by | J. Stokes |
| Date of Accession | 02/02/1964 |
| History | N.G.Heatley |
| Other collection IDs | ATCC9144 CCM2107 CIP53.154 CN6457 DSM683 NCTC6571 NRRLB-3141 NCDO907 |
| WDCM number | WDCM 00035 |
| Yeast? | False |
| K12 | No |
| References | Heatley N.G. (1944) Biochem J. 38 p61. A method for the assay of penicillin Cook A.M. et al (1956) J. Pharm. Pharmacol. 8 p721 Hugo W.B. and Stretton R.G. (1964) Nature 202 p1217. Action of quinacillin on Staphylococcus aureus. Silvestri L.G. and Hill L.R. (1965) J. Bact. 90 p136. Agreement between DNA base composition and taxometric classification of gram positive bacteria. Allwood M.C. and Russell R.D. (1966) Lab. Pract. 15 p1132. The storage of washed suspensions of Staphylococcus aureus. Roscoe J. and Abraham E.P. (1966) Biochem J. 99 p793. Experiments relating to the biosynthesis of bacilysin Watts F.W.C. (1967) J. med. Lab. Tech. 24 p129. Rules and Regulations "Antibiotic susceptibility discs" Federal Register 37 20525-20529 (1972) Bloomfield S. (1974) J. appl. Bact. 37 p117 Owens J.J. (1974) J. appl. Bact. 37 p137 Dastidar S.G. et al (1974) J. gen. Microbiol. 84 p245 Coyle M.B. et al (1976) Antimicrobial Agents Chemother. 10 pp436-440. Reproducibility of control strains for antibiotic susceptibility testing WHO Expert Committee on Biological Standardization 28 Report. Technical Report Series 610, Genf 1977. Requirements for antibiotic susceptibility tests. I. Agar diffusion tests using antibiotic susceptibility discs. Code of Federal Regulations title 21 part 460 1977 & 1978 National Committee on clinical laboratory standards. Performance standards for antimicrobial disc susceptibility test. Approved standard ASM-2, Villanova Ps (1979) Lenelter E.H. et al Manual of Clinical Microbiology (3rd ed.) American Society for Microbiology, Washington (1980) Sourcebook of Experiments for the Teaching of Microbiology, Eds. S.B. Primrose and A.C. Wardlaw, Academic Press 1982. [Expt. 46, 73] J. appl. Bact. (1985) 59 pp537-543 (Unknown), 34 J.Clin.Microbiol., 1995 (Unknown), 90 J.Antimicrob.Chemother., 1994 (Unknown), Analytical Microbiology J.Bacteriol., 1965, 1972 AOAC International, Chlortetracycline HCl in feeds, turbidimetric method Gaithersburg, MD, AOAC International; AOAC Official Method 977.37. U, S Pharmacopeia Antibiotics--microbial assays In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 1690 U, S Pharmacopeia Antibiotics--Microbial assays In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1996 Iodophors for use in the dairying industry.Sydney, NSW, Australia, (journal unknown) , Standards Australia AS 1398-1998 A method for the assay of penicillin, Biochem J 38(1), 61-65, 1944 Agreement Between Deoxyribonucleic Acid Base Composition and Taxometric Classification of Gram-Positive Cocci, J Bacteriol 90(1), 136-140, 1965 Beta-lactam resistance in Staphylococcus aureus cells that do not require a cell wall for integrity, Antimicrob Agents Chemother 49(12), 5075-5080, 2005 British Pharmacopoeia Commission, Biological assay of antibiotics In: British Pharmacopoeia 1993 vol 2: London (UK), British Pharmacopoeia Commission; 1993 European Pharmacopoeia Commission, Microbiological assay of antibiotics In: European Pharmacopoeia 3rd ed Strasbourg, France, European Pharmacopoeia Commission; 1997 European Pharmacopoeia. 2.7.2 Antibiotica assays, (journal unknown) , 85-90, 2000 Identification of a novel gene cluster encoding staphylococcal exotoxin-like proteins: characterization of the prototypic gene and its protein product, SET1, Infect Immun 68(8), 4407-4415, 2000 Kavanagh, F. (ed.). Analytical Microbiology, vol. II. Academic Press, New York 1972, (journal unknown) , 1972 Microbial assay for antibiotics.Tokyo, Japan, (journal unknown) , Japanese Pharmacopoeia Rapid screening for putative exported proteins from Staphylococcus aureus using alkaline phosphatase as a reporter molecule, Mol Biotechnol 15(1), 11-20, 2000 Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting, Electrophoresis 32(21), 2951-2965, 2011 Staphylococcus aureus subsp. anaerobius subsp. nov., the causal agent of abscess disease of sheep, Int J Syst Bacteriol 35, 99-102, 1985 Water microbiology. Method 16: Bactericidal efficiency of water disinfecting tablets.Sydney, NSW, Australia: Standards Australia, (journal unknown) , Standards Australia AS/NZS 4276.16, 1999 Water microbiology. Method 20: Examination for coagulase positive staphylococci, including Staphylococcus aureus, by memebrane filtration.Sydney, NSW, Australia: Standards Australia, (journal unknown) , Standards Australia AS/NZS 4276.20, 2003 |