| NCIMB number | NCIMB 9518 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Staphylococcus aureus subsp. aureus |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 2 days |
| Growth factors (and/or information) | Lab Lemco, 37C, 24hr: good growth, butyrous consistency, easily emulsified suspension, discrete differentiation |
| ACDP category | 2 |
| Hazard information | This species has been associated with food poisoning, furuncles, impetigo, pneumonia, mastitis, enterocolitis, urogenital infections, toxic shock syndrome, meningitis, endocarditis, toxic epidermal necrolysis and various abscesses. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Golden |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | 0.9 u approx |
| Cellular Arrangement | Irregular Clusters |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Name | T.G.Mitchell |
| Depositor Address | |
| Source | Human lesion |
| Date of Accession | 01/01/1964 |
| History | "Diversey(UK) Ltd. -- Diversey Corp., Chicago -- ATCC -- FDA -- AMC" |
| Other collection IDs | ATCC6538 CIP4.83 DSM799 FDA209 IFO13276 IMET10761 NCTC10788 NRBC13276 |
| WDCM number | WDCM 00032 |
| Yeast? | False |
| K12 | No |
| References | Reddish G.F. (1929) J. Lab. Clin. Med. 14 p649 Klarmann E.G. and Wright E.S. (1948) Am. J. Pharm. 120 p146 Klarmann E.G. and Wright E.S. (1950) Am. J. Pharm. 122 p5 Cantor A. and Shelanski H.A. (1951) Soap NY 27 (2) p133 Stuart L.S. et al (1953) J. Ass. Off. Agric. Chem. 36 p466 Stuart L.S. (1954) "Antiseptics, Disinfectants, Fungicides, and chemical and physical sterilization" London: Henry Kimpton, ed by G.F. Reddish. Chambers C.W. (1956) J. Milk Fd Technol. 19 p183 Mitchell T.G. (1963) J. med. Lab. Technol. 20 p269 Am. Assoc. Textile Colourists and Chemists (AATCC) Technical Manual (1966) pp B-157. "Tentative Test Method AATCC 90-1965T, Antibacterial activity of fabrics, detection of: agar plate method." Am. Assoc Textile Colourists and chemists (AATCC) Technical Manual (1966) ppB-158 "Antibacterial finishes on fabrics Evaluation of " Tentative Test Method AATCC100-1965T. British Pharmacopoeia (1980) Addendum 1986 Tests for Microbial Contamination A104 Appendix XIV and A109 Appendix XIV. Efficacy of Antimicrobial Preservatives in Pharmaceutical Products. MAFF, FACC, Reveiw of Remaining Classes of Food Additives used as Ingredients in Food. Report on the review of enzyme preparations 1982. (Unknown), Zentralbl Bakteriol 160, 342, 1975 (Unknown), Zentralbl Bakteriol 172, 534, 1981 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11133-2, 2003 U, S Pharmacopeia Microbial limit tests -- Nutritional supplements -- Preparatory testing In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 2180 AOAC International, Bacteriostatic activity of laundry additive disinfectants Gaithersburg, MD, AOAC International; AOAC Official Method 972.04, 1990 AOAC International, Germicidal and detergent sanitizing action of disinfectants Gaithersburg, MD, AOAC International; AOAC Official Method 960.09, 1990 Evaluation of Reduced Susceptibility to Quaternary Ammonium Compounds and Bisbiguanides in Clinical Isolates and Laboratory-Generated Mutants of Staphylococcus aureus, Antimicrob Agents Chemother 57(8), 3488-3497, 2013 Evaluation of the repeatability and reproducibility of European suspension test methods for antimicrobial activity of disinfectants and antiseptics, J Appl Bacteriol 73(1), 87-93, 1992 Richtlinien für die Prüfung und Bewertung chemischer Desinfektionsverfahren, Zentralbl Bakteriol Hyg I Abt Orig B 172, 534-562, 1981 Air Sanitizers.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-11, 1980 Carpet Sanitizers.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-8, 1981 Chemical disinfectants and antiseptics -- Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1).London, UK: British Standards Institution; British Standard BS EN 1276, (journal unknown) , 1997, 1997 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (Phase 1).London, UK: British Standards Institution; British Standard BS EN 1040, (journal unknown) , 2005, 2005 Confimatory Efficacy Data Requirements.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-5, 1982 Disinfectants for Use on Hard Surfaces.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-1, 1982 Laundry Additives--Disinfection and Saitization.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-13, 1980 London, UK: British Standards Institution, (journal unknown) , British Standard BS EN 13727, 2003 Laundry Additives- Residual Self-Sanitization.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-14, 1981 A novel resistance mechanism to triclosan that suggests horizontal gene transfer and demonstrates a potential selective pressure for reduced biocide susceptibility in clinical strains of Staphylococcus aureus, Int J Antimicrob Agents 40(3), 210-220, 2012 A theoretical approach of disinfectant testing, Zentralbl Bakteriol Parasitenkd OrigB Abt I 160, 342-367, 1975 European Pharmacopeia. 2.6.1. Sterility Testing. 2.6.12. and 2.6.13 Microbiological testing of non-sterile products, (journal unknown) , 51-65, 2000 Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14730, 2000 Ophthalmic optics--Contact lens care products--Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14729, 2001 Staphylococcus aureus subsp. anaerobius subsp. nov., the causal agent of abscess disease of sheep, Int J Syst Bacteriol 35, 99-102, 1985 The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products, J Appl Bacteriol 76(1), 68-74, 1994 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/25627827https://www.ncbi.nlm.nih.gov/m/pubmed/22247408 |
| NCIMB number | NCIMB 9518 |
| Deposit type | Bacteria |
| Type strain | No |
| GMO | No |
| Taxon name | Staphylococcus aureus subsp. aureus |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Incubation period | 2 days |
| Growth factors (and/or information) | Lab Lemco, 37C, 24hr: good growth, butyrous consistency, easily emulsified suspension, discrete differentiation |
| ACDP category | 2 |
| Hazard information | This species has been associated with food poisoning, furuncles, impetigo, pneumonia, mastitis, enterocolitis, urogenital infections, toxic shock syndrome, meningitis, endocarditis, toxic epidermal necrolysis and various abscesses. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Shiny |
| Colony Shape | Circular |
| Colony Elevation | Convex |
| Colony Colour | Golden |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | 0.9 u approx |
| Cellular Arrangement | Irregular Clusters |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Name | T.G.Mitchell |
| Depositor Address | |
| Source | Human lesion |
| Date of Accession | 01/01/1964 |
| History | "Diversey(UK) Ltd. -- Diversey Corp., Chicago -- ATCC -- FDA -- AMC" |
| Other collection IDs | ATCC6538 CIP4.83 DSM799 FDA209 IFO13276 IMET10761 NCTC10788 NRBC13276 |
| WDCM number | WDCM 00032 |
| Yeast? | False |
| K12 | No |
| References | Reddish G.F. (1929) J. Lab. Clin. Med. 14 p649 Klarmann E.G. and Wright E.S. (1948) Am. J. Pharm. 120 p146 Klarmann E.G. and Wright E.S. (1950) Am. J. Pharm. 122 p5 Cantor A. and Shelanski H.A. (1951) Soap NY 27 (2) p133 Stuart L.S. et al (1953) J. Ass. Off. Agric. Chem. 36 p466 Stuart L.S. (1954) "Antiseptics, Disinfectants, Fungicides, and chemical and physical sterilization" London: Henry Kimpton, ed by G.F. Reddish. Chambers C.W. (1956) J. Milk Fd Technol. 19 p183 Mitchell T.G. (1963) J. med. Lab. Technol. 20 p269 Am. Assoc. Textile Colourists and Chemists (AATCC) Technical Manual (1966) pp B-157. "Tentative Test Method AATCC 90-1965T, Antibacterial activity of fabrics, detection of: agar plate method." Am. Assoc Textile Colourists and chemists (AATCC) Technical Manual (1966) ppB-158 "Antibacterial finishes on fabrics Evaluation of " Tentative Test Method AATCC100-1965T. British Pharmacopoeia (1980) Addendum 1986 Tests for Microbial Contamination A104 Appendix XIV and A109 Appendix XIV. Efficacy of Antimicrobial Preservatives in Pharmaceutical Products. MAFF, FACC, Reveiw of Remaining Classes of Food Additives used as Ingredients in Food. Report on the review of enzyme preparations 1982. (Unknown), Zentralbl Bakteriol 160, 342, 1975 (Unknown), Zentralbl Bakteriol 172, 534, 1981 Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media..Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 11133-2, 2003 U, S Pharmacopeia Microbial limit tests -- Nutritional supplements -- Preparatory testing In: US Pharmacopeia 23rd rev Rockville, MD, U.S. Pharmacopeia; 1995, 2180 AOAC International, Bacteriostatic activity of laundry additive disinfectants Gaithersburg, MD, AOAC International; AOAC Official Method 972.04, 1990 AOAC International, Germicidal and detergent sanitizing action of disinfectants Gaithersburg, MD, AOAC International; AOAC Official Method 960.09, 1990 Evaluation of Reduced Susceptibility to Quaternary Ammonium Compounds and Bisbiguanides in Clinical Isolates and Laboratory-Generated Mutants of Staphylococcus aureus, Antimicrob Agents Chemother 57(8), 3488-3497, 2013 Evaluation of the repeatability and reproducibility of European suspension test methods for antimicrobial activity of disinfectants and antiseptics, J Appl Bacteriol 73(1), 87-93, 1992 Richtlinien für die Prüfung und Bewertung chemischer Desinfektionsverfahren, Zentralbl Bakteriol Hyg I Abt Orig B 172, 534-562, 1981 Air Sanitizers.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-11, 1980 Carpet Sanitizers.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-8, 1981 Chemical disinfectants and antiseptics -- Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1).London, UK: British Standards Institution; British Standard BS EN 1276, (journal unknown) , 1997, 1997 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (Phase 1).London, UK: British Standards Institution; British Standard BS EN 1040, (journal unknown) , 2005, 2005 Confimatory Efficacy Data Requirements.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-5, 1982 Disinfectants for Use on Hard Surfaces.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-1, 1982 Laundry Additives--Disinfection and Saitization.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-13, 1980 London, UK: British Standards Institution, (journal unknown) , British Standard BS EN 13727, 2003 Laundry Additives- Residual Self-Sanitization.Washington, DC: Environmental Protection Agency, (journal unknown) , EPA EPA DIS/TSS-14, 1981 A novel resistance mechanism to triclosan that suggests horizontal gene transfer and demonstrates a potential selective pressure for reduced biocide susceptibility in clinical strains of Staphylococcus aureus, Int J Antimicrob Agents 40(3), 210-220, 2012 A theoretical approach of disinfectant testing, Zentralbl Bakteriol Parasitenkd OrigB Abt I 160, 342-367, 1975 European Pharmacopeia. 2.6.1. Sterility Testing. 2.6.12. and 2.6.13 Microbiological testing of non-sterile products, (journal unknown) , 51-65, 2000 Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14730, 2000 Ophthalmic optics--Contact lens care products--Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses.Geneva (Switzerland): International Organization for Standardization/ANSI, (journal unknown) , ISO ISO 14729, 2001 Staphylococcus aureus subsp. anaerobius subsp. nov., the causal agent of abscess disease of sheep, Int J Syst Bacteriol 35, 99-102, 1985 The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products, J Appl Bacteriol 76(1), 68-74, 1994 |
| PubMed Publication | https://www.ncbi.nlm.nih.gov/m/pubmed/25627827https://www.ncbi.nlm.nih.gov/m/pubmed/22247408 |