| NCIMB number | NCIMB 8711 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Staphylococcus saprophyticus subsp. saprophyticus |
| Depositor designation | S41 |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | NA, 30C, 48hr: good growth |
| ACDP category | 2 |
| Hazard information | This species has been associated with urinary tract infections and prostatitis. Although not currently (2004) listed as ACDP Group 2, we recommend that cultures be handled at this containment level. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | White |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | < 1 u |
| Cellular Arrangement | Pairs/Chains/Cluster |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Source | Urine |
| Date of Accession | 01/11/1955 |
| History | S.T.Cowan |
| Other collection IDs | ATCC15305 ATCC19701 CCM883 CIP76.125 DSM20229 IAM12452 NCTC7292 NCDO948 |
| Yeast? | False |
| K12 | No |
| References | Fairbrother (1940) J. path. Bact. 50 p83 Shaw C. et al (1951) J. gen. Microbiol. 5 p1010 Silvestri L.G. and Hill L.R. (1965) J. Bact. 90 p136 Schleifer K.H. and Kocur M. (1973) Arch. Mikrobiol. 93 pp65-85. IJSB 46:793 IJSEM (2003) 53:88 Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis, Vet Microbiol 134(3-4), 327-333, 2009 Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species, J Clin Microbiol 42(11), 4988-4995, 2004 Kinship in the SRP RNA family, RNA Biol 6(5), 508-516, 2009 The tmRDB and SRPDB resources, Nucleic Acids Res 34(Databaseissue), D163-D168, 2006 The tmRNA website: reductive evolution of tmRNA in plastids and other endosymbionts, Nucleic Acids Res 32(Databaseissue), D104-D108, 2004 Biofilm formation and the presence of the intercellular adhesion locus ica among staphylococci from food and food processing environments, Appl Environ Microbiol 69(9), 5648-5655, 2003 Comparative study using various methods for identification of Staphylococcus species in clinical specimens., J Clin Microbiol 44(8), 2824-2830, 2006 Contrasting immunological effects of two disparate dusts - preliminary observations, Int Arch Allergy Immunol 149(1), 81-90, 2009 Development of a PCR assay for identification of staphylococci at genus and species levels, J Clin Microbiol 39(7), 2541-2547, 2001 Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples, J Clin Microbiol 38(9), 3280-3284, 2000 Differentiation of Staphylococcus spp. by terminal-restriction fragment length polymorphism analysis of glyceraldehyde-3-phosphate dehydrogenase-encoding gene, J Microbiol Methods 70(3), 542-549, 2007 dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus, Int J Syst Evol Microbiol 57, 25-30, 2007 Gas-liquid chromatography of cellular fatty acids for identification of staphylococci, J Clin Microbiol 32(8), 1908-1910, 1994 HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci, J Clin Microbiol 34(4), 818-823, 1996 Identification of staphylococci and dominant lactic acid bacteria in spontaneously fermented Swiss meat products using PCR-RFLP, Food Microbiol 29(2), 157-166, 2012 Identification of Staphylococcus carnosus strains from fermented fish and soy sauce mash, J Gen Appl Microbiol 37, 479-494, 1991 Identification of the Staphylococcus sciuri species group with EcoRI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov, Int J Syst Bacteriol 44(3), 454-460, 1994 Notification that new names and new combinations have appeared in volume 46, no. 3, of the IJSB, Int J Syst Bacteriol 46, 1191, 1996 Phylogenetic analyses of Staphylococcus based on the 16S rDNA sequence and assignment of clinical isolates from animals, J Vet Med Sci 59(9), 775-783, 1997 Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target, J Clin Microbiol 39(12), 4296-4301, 2001 rpoB gene sequence-based identification of Staphylococcus species, J Clin Microbiol 40(4), 1333-1338, 2002 Species identification and phylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus, Int J Syst Bacteriol 49 Pt 3, 1181-1192, 1999 Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Eur J Clin Microbiol Infect Dis 30(3), 343-354, 2011 Topoisomerase sequences of coagulase-negative staphylococcal isolates resistant to ciprofloxacin or trovafloxacin, Antimicrob Agents Chemother 43(7), 1631-1637, 1999 Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection, Proc Natl Acad Sci U S A 102(37), 13272-13277, 2005 |
| NCIMB number | NCIMB 8711 |
| Deposit type | Bacteria |
| Type strain | Yes |
| GMO | No |
| Taxon name | Staphylococcus saprophyticus subsp. saprophyticus |
| Depositor designation | S41 |
| Preservation method | Lyophilised |
| Price band | A |
| Media | 001 |
| Gas regime | aerobic |
| Growth factors (and/or information) | NA, 30C, 48hr: good growth |
| ACDP category | 2 |
| Hazard information | This species has been associated with urinary tract infections and prostatitis. Although not currently (2004) listed as ACDP Group 2, we recommend that cultures be handled at this containment level. |
| Colony Edge | Entire |
| Colony Surface | Smooth/Moist |
| Colony Shape | Circular |
| Colony Elevation | Flat |
| Colony Colour | White |
| Colony Opacity | Opaque |
| Cellular Shape | Coccus |
| Cellular Size | < 1 u |
| Cellular Arrangement | Pairs/Chains/Cluster |
| Cellular Motility | No |
| Gram stain | Gram Positive |
| Depositor Company | National Collection of Type Cultures (NCTC) |
| Depositor Address | 61 Colindale Avenue London U.K. NW9 5HT |
| Source | Urine |
| Date of Accession | 01/11/1955 |
| History | S.T.Cowan |
| Other collection IDs | ATCC15305 ATCC19701 CCM883 CIP76.125 DSM20229 IAM12452 NCTC7292 NCDO948 |
| Yeast? | False |
| K12 | No |
| References | Fairbrother (1940) J. path. Bact. 50 p83 Shaw C. et al (1951) J. gen. Microbiol. 5 p1010 Silvestri L.G. and Hill L.R. (1965) J. Bact. 90 p136 Schleifer K.H. and Kocur M. (1973) Arch. Mikrobiol. 93 pp65-85. IJSB 46:793 IJSEM (2003) 53:88 Comparison of a commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequence-based genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis, Vet Microbiol 134(3-4), 327-333, 2009 Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species, J Clin Microbiol 42(11), 4988-4995, 2004 Kinship in the SRP RNA family, RNA Biol 6(5), 508-516, 2009 The tmRDB and SRPDB resources, Nucleic Acids Res 34(Databaseissue), D163-D168, 2006 The tmRNA website: reductive evolution of tmRNA in plastids and other endosymbionts, Nucleic Acids Res 32(Databaseissue), D104-D108, 2004 Biofilm formation and the presence of the intercellular adhesion locus ica among staphylococci from food and food processing environments, Appl Environ Microbiol 69(9), 5648-5655, 2003 Comparative study using various methods for identification of Staphylococcus species in clinical specimens., J Clin Microbiol 44(8), 2824-2830, 2006 Contrasting immunological effects of two disparate dusts - preliminary observations, Int Arch Allergy Immunol 149(1), 81-90, 2009 Development of a PCR assay for identification of staphylococci at genus and species levels, J Clin Microbiol 39(7), 2541-2547, 2001 Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples, J Clin Microbiol 38(9), 3280-3284, 2000 Differentiation of Staphylococcus spp. by terminal-restriction fragment length polymorphism analysis of glyceraldehyde-3-phosphate dehydrogenase-encoding gene, J Microbiol Methods 70(3), 542-549, 2007 dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus, Int J Syst Evol Microbiol 57, 25-30, 2007 Gas-liquid chromatography of cellular fatty acids for identification of staphylococci, J Clin Microbiol 32(8), 1908-1910, 1994 HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci, J Clin Microbiol 34(4), 818-823, 1996 Identification of staphylococci and dominant lactic acid bacteria in spontaneously fermented Swiss meat products using PCR-RFLP, Food Microbiol 29(2), 157-166, 2012 Identification of Staphylococcus carnosus strains from fermented fish and soy sauce mash, J Gen Appl Microbiol 37, 479-494, 1991 Identification of the Staphylococcus sciuri species group with EcoRI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov, Int J Syst Bacteriol 44(3), 454-460, 1994 Notification that new names and new combinations have appeared in volume 46, no. 3, of the IJSB, Int J Syst Bacteriol 46, 1191, 1996 Phylogenetic analyses of Staphylococcus based on the 16S rDNA sequence and assignment of clinical isolates from animals, J Vet Med Sci 59(9), 775-783, 1997 Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target, J Clin Microbiol 39(12), 4296-4301, 2001 rpoB gene sequence-based identification of Staphylococcus species, J Clin Microbiol 40(4), 1333-1338, 2002 Species identification and phylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus, Int J Syst Bacteriol 49 Pt 3, 1181-1192, 1999 Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Eur J Clin Microbiol Infect Dis 30(3), 343-354, 2011 Topoisomerase sequences of coagulase-negative staphylococcal isolates resistant to ciprofloxacin or trovafloxacin, Antimicrob Agents Chemother 43(7), 1631-1637, 1999 Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection, Proc Natl Acad Sci U S A 102(37), 13272-13277, 2005 |