一、菌种简介
1、菌种名称：球孢白僵菌 Beauveria bassiana
2、菌种编号：TS729680
3、生物危害程度：四类
二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。
三、培养条件
1、培养基： 马铃薯、葡萄糖琼脂培养基(PDA)
2、培养基成分：马铃薯提取液 1.0L，葡萄糖 20.0g，琼脂 15.0g，pH自然。[注] 马铃薯提取液：取去皮马铃薯200g，切成小块， 加水1.0L煮沸30min，滤去马铃薯块，将滤液补足至1.0L。可以使用商品化成品培养基。
3、需氧类型：好氧
4、培养温度：25
四、注意事项
1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。
2、试管斜面菌种请尽快转接，不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，CICC不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
References[edit]
^ Jump up to:a b Barbarin, Alexis M.; Jenkins, Nina E.; Rajotte, Edwin G.; Thomas, Matthew B. (15 September 2012). "A preliminary evaluation of the potential of Beauveria bassiana for bed bug control" (PDF). Journal of Invertebrate Pathology. 111 (1): 82–85. doi:10.1016/j.jip.2012.04.009. PMID 22555012.
^ Jump up to:a b Donald G. McNeil Jr., Fungus Fatal to Mosquito May Aid Global War on Malaria, The New York Times, 10 June 2005
^ Zimmermann, Gisbert (2007). "Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii". Biocontrol Science and Technology. Taylor & Francis. 17 (6): 553–596. doi:10.1080/09583150701309006. ISSN 0958-3157. S2CID 85350953. p. 556: Biological properties of Beauveria spp.
History
...The early history of B. bassiana started in 1835. It was Agostino Bassi di Lodi from Italy... He observed a disease in silkworms, Bombyx mori, which he called 'white muscardine' and started the first infection experiments. The fungus was then studied and described by the famous Italian naturalist Giuseppe Gabriel Balsamo-Crivelli in 1835, who gave it the name Botrytis bassiana, in honour of Bassi (Steinhaus 1949; Müller-Kögler 1965; Rehner 2005).
In 1911, Beauverie studied the fungus again and in 1912 Vuillemin created the new genus Beauveria in honor of Beauverie, of which the species B. bassiana became the type.^ Rehner SA, Buckley E (2005). "A Beauveria phylogeny inferred from nuclear ITS and EF1-{alpha} sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs". Mycologia. 97 (1): 84–98. doi:10.3852/mycologia.97.1.84. PMID 16389960.
^ Rehner, Stephen A.; Minnis, Andrew M.; Sung, Gi-Ho; Luangsaard, J. Jennifer; Devotto, Luis; Humber, Richard A. (2011). "Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria". Mycologia. 103 (5): 1055–1073. doi:10.3852/10-302. PMID 21482632. S2CID 39902951.
^ Li ZZ, Li CR, Huang B, Fan MZ (2001). "Discovery and demonstration of the teleomorph of Beauveria bassiana (Bals.) Vuill., an important entomogenous fungus". Chinese Science Bulletin. 46 (9): 751–3. Bibcode:2001ChSBu..46..751L. doi:10.1007/BF03187215. S2CID 94635367.
^ Posada, F.; et al. (2004). "Syspastospora parasitica, a mycoparasite of the fungus Beauveria bassiana attacking the Colorado potato beetle Leptinotarsa decemlineata: a tritrophic association". J. Insect Sci. 4: 24. doi:10.1093/jis/4.1.24. PMC 528884. PMID 15861239.
^ "EPA Factsheet". Retrieved 2006-12-14.
^ "Cornell Extension Service". Archived from the original on 13 December 2006. Retrieved 2006-12-14.
^ "University of Connecticut Extension". Archived from the original on 2006-09-01. Retrieved 2006-12-14.
^ "University of Minnesota Extension". Archived from the original on 7 December 2006. Retrieved 2006-12-14.
^ Gouli, Vladimir; Gouli, Svetlana; Skinner, Margaret; Hamilton, George; Kim, Jae Sue; Parker, Bruce L (February 2012). "Virulence of select entomopathogenic fungi to the brown marmorated stink bug, Halyomorpha halys (Stål) (Heteroptera: Pentatomidae)". Pest Management Science. 68 (2): 155–157. doi:10.1002/ps.2310. PMID 22223199.
^ Mann, Andrew; Davis, Thomas (2020). "Plant secondary metabolites and low temperature are the major limiting T factors for Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales) growth and virulence in a bark beetle system". Biological Control. 141: 104130. doi:10.1016/j.biocontrol.2019.104130. S2CID 209582862.
^ "Biocontrol: Fungus and Wasps Released to Control Emerald Ash Borer". Science News. ScienceDaily. 26 April 2011. Retrieved 3 February 2012.
^ Un hongo acaba con el 75% del picudo rojo que afecta al conjunto del Palmeral de Elche (in Spanish)
^ Cossentine, J. E.; Judd, G. J. R.; Bissett, J. D.; Lacey, L. A. (2010-01-01). "Susceptibility of apple clearwing moth larvae, Synanthedon myopaeformis (Lepidoptera: Sesiidae) to Beauveria bassiana and Metarhizium brunneum". Biocontrol Science and Technology. 20 (7): 703–707. doi:10.1080/09583151003690390. ISSN 0958-3157. S2CID 84565528.
^ Tucker DL, Beresford CH, Sigler L, Rogers K (November 2004). "Disseminated Beauveria bassiana infection in a patient with acute lymphoblastic leukemia". J. Clin. Microbiol. 42 (11): 5412–4. doi:10.1128/JCM.42.11.5412-5414.2004. PMC 525285. PMID 15528759.
^ Wagner BL, Lewis LC (August 2000). "Colonization of corn, Zea mays, by the entomopathogenic fungus Beauveria bassiana". Appl. Environ. Microbiol. 66 (8): 3468–73. Bibcode:2000ApEnM..66.3468W. doi:10.1128/AEM.66.8.3468-3473.2000. PMC 92172. PMID 10919808.
^ Jensen, J. M.; Robinson, B. E.; Bulmer, G. S. (July 1979). "Fatal Mycotic Pulmonary Disease of Captive American Alligators". Veterinary Pathology. 16 (4): 428–431. doi:10.1177/030098587901600405. ISSN 0300-9858. PMID 452316. S2CID 24883485.
^ Georg, Lucille K.; Williamson, W.M.; Tilden, Evelyn B.; Getty, Ruth E. (1962). "Mycotic pulmonary disease of captive giant tortoises due toBeauvaria bassianaandPaecilomyces fumoso-roseus". Medical Mycology. 2 (2): 80–86. doi:10.1080/00362176385190161. ISSN 1369-3786.
^ Cabo, J. F. González; Serrano, J. Espejo; Asensio, M. C. Bárcena (March 1995). "Mycotic pulmonary disease byBeauveria bassianain a captive tortoise". Mycoses. 38 (3–4): 167–169. doi:10.1111/j.1439-0507.1995.tb00043.x. ISSN 0933-7407. PMID 7477096. S2CID 85398656.
^ Müller-Kögler, E. (December 1967). "Nebenwirkungen Insektenpathogener Pilze Auf Mensch und Wirbeltiere: Aktuelle Fragen". Entomophaga. 12 (4): 429–441. doi:10.1007/bf02376929. ISSN 0013-8959. S2CID 44795822.
^ Barbarin, Alexis (20 March 2017). "Susceptibility of insecticide-resistant bed bugs (Cimex lectularius) to infection by fungal biopesticide". Pest Management Science. 73 (8): 1568–1573. doi:10.1002/ps.4576. PMC 5518228. PMID 28321982.
^ Dubovskiy, Ivan M.; Whitten, Miranda M. A.; Yaroslavtseva, Olga N.; Greig, Carolyn; Kryukov, Vadim Y.; Grizanova, Ekaterina V.; Mukherjee, Krishnendu; Vilcinskas, Andreas; Glupov, Viktor V.; Butt, Tariq M. (2013-04-01). Leulier, François (ed.). "Can Insects Develop Resistance to Insect Pathogenic Fungi?". PLoS ONE. Public Library of Science. 8 (4): e60248. Bibcode:2013PLoSO...860248D. doi:10.1371/journal.pone.0060248. ISSN 1932-6203. PMC 3613352. PMID 23560083. S2CID 15239538.
^ "Genetically modified fungus leaked". 3 News NZ. March 20, 2013. Archived from the original on January 15, 2014. Retrieved March 20, 2013.
