一、菌种简介
1、菌种名称：凝结芽胞杆菌 Bacillus coagulans
2、菌种编号：TS729670
3、生物危害程度：四类
二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。
三、培养条件
1、培养基： 营养肉汁琼脂
2、培养基成分：蛋白胨 5.0g，牛肉浸取物 3.0g，NaCl 5.0g，琼脂 15.0g，蒸馏水 1.0L，pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4∙H2O，则有利于产生芽孢。推荐使用商品化成品培养基。
3、需氧类型：好氧
4、培养温度：37
四、注意事项
1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。
2、试管斜面菌种请尽快转接，不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
References[edit]
Hong, H. A.; Duc, L. H.; Cutting, S. M. (2005). "The use of bacterial spore formers as probiotics". FEMS Microbiology Reviews. 29 (4): 813–835. doi:10.1016/j.femsre.2004.12.001. PMID 16102604. Archived from the original on 2009-08-01.
Notes[edit]
^ Hammer, B. W. 1915. Bacteriological studies on the coagulation of evaporated milk. Iowa Agric. Exp. Stn. Res. Bull. 19:119-131
^ Jump up to:a b "Lactobacillus sporogenes a probiotioc species ?". www.food-info.net.
^ "Official list of bacterial names". Archived from the original on 2007-10-30. Retrieved 2007-11-02.
^ Gupta, Radhey S.; Patel, Sudip; Saini, Navneet; Chen, Shu (2020-11-01). "Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species". International Journal of Systematic and Evolutionary Microbiology. 70 (11): 5753–5798. doi:10.1099/ijsem.0.004475. ISSN 1466-5026. PMID 33112222.
^ "The maintenance of the list of QPS microorganisms intentionally added to food or feed". Scientific Opinion of the Panel on Biological Hazards.
^ Sanders, M. E.; Morelli, L.; Tompkins, T. A. (2003). "Sporeformers as Human Probiotics: Bacillus, Sporolactobacillus, and Brevibacillus". Comprehensive Reviews in Food Science and Food Safety. 2 (3): 101–110. doi:10.1111/j.1541-4337.2003.tb00017.x. PMID 33451235.
^ "LACTOBACILLUS SPOROGENES OR BACILLUS COAGULANS: MISIDENTIFICATION OR MISLABELLING?" (PDF). Archived from the original (PDF) on 2010-11-05. Retrieved 2007-01-16.
^ Hun, L. (2009). "Bacillus coagulans significantly improved abdominal pain and bloating in patients with IBS". Postgraduate Medicine. 121 (2): 119–124. doi:10.3810/pgm.2009.03.1984. PMID 19332970. S2CID 7698543.
^ Baron, M. (2009). "A patented strain of Bacillus coagulans increased immune response to viral challenge". Postgraduate Medicine. 121 (2): 114–118. doi:10.3810/pgm.2009.03.1971. PMID 19332969. S2CID 38408989.
^ Fitzpatrick, LR. (Aug 2013). "Probiotics for the treatment of Clostridium difficile associated disease". World J Gastrointest Pathophysiol. 4 (3): 47–52. doi:10.4291/wjgp.v4.i3.47. PMC 3740259. PMID 23946887.
^ Sadeghirashed, Saba; Kazemi, Fatemeh; Taheri, Saba; Ebrahimi, Maryam Tajabadi; Arasteh, Javad (2021-11-10). "A novel probiotic strain exerts therapeutic effects on mouse model of multiple sclerosis by altering the expression of inflammasome and IDO genes and modulation of T helper cytokine profile". Metabolic Brain Disease. 37 (1): 197–207. doi:10.1007/s11011-021-00857-7. ISSN 0885-7490. PMID 34757579. S2CID 243939724.
^ Endres, J. R.; Clewell, A.; Jade, K. A.; Farber, T.; Hauswirth, J.; Schauss, A. G. (2009). "Safety assessment of a proprietary preparation of a novel Probiotic, Bacillus coagulans, as a food ingredient". Food and Chemical Toxicology. 47 (6): 1231–1238. doi:10.1016/j.fct.2009.02.018. PMC 2726964. PMID 19248815.
^ Bomko TV, Nosalskaya TN, Kabluchko TV, Lisnyak YV, Martynov AV (2017). "Immunotropic aspect of the Bacillus coagulans probiotic action". Journal of Pharmacy and Pharmacology. 69 (8): 1033–40. doi:10.1111/jphp.12726. PMID 28397382. S2CID 4738876.
^ Sanders, Mary Ellen; Morelli, Lorenzo; Bush, Scott (14 August 2001). "Lactobacillus sporogenes Is Not a Lactobacillus Probiotic". ASM News. 67 (8). Archived from the original on 2013-05-20. Retrieved 2022-09-10.
