一、菌种简介
1、菌种名称：费氏柠檬酸杆菌 Citrobacter freundii
2、菌种编号：TS724161
3、生物危害程度：四类
二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。
三、培养条件
1、培养基： 营养肉汁琼脂
2、培养基成分：蛋白胨 5.0g，牛肉浸取物 3.0g，NaCl 5.0g，琼脂 15.0g，蒸馏水 1.0L，pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4∙H2O，则有利于产生芽孢。推荐使用商品化成品培养基。
3、需氧类型：好氧
4、培养温度：30
四、注意事项
1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。
2、试管斜面菌种请尽快转接，不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
References[edit]
^ Jump up to:a b c d Choudhuri, Indranil; Khanra, Kalyani; Maity, Prasenjit; Patra, Anutosh; Maity, Gajendra Nath; Pati, Bikas Ranjan; Nag, Anish; Mondal, Soumitra; Bhattacharyya, Nandan (2021). "Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii". International Journal of Biological Macromolecules. 168: 537–549. doi:10.1016/j.ijbiomac.2020.12.063. PMID 33316341. S2CID 229180023.
^ Jump up to:a b c d e f g Ramos-Vivas, José; Chapartegui-González, Itziar; Fernández-Martínez, Marta; González-Rico, Claudia; Barrett, John; Fortún, Jesús; Escudero, Rosa; Marco, Francesc; Linares, Laura; Nieto, Javier; Aranzamendi, Maitane (2020). "Adherence to Human Colon Cells by Multidrug Resistant Enterobacterales Strains Isolated From Solid Organ Transplant Recipients With a Focus on Citrobacter freundii". Frontiers in Cellular and Infection Microbiology. 10: 447. doi:10.3389/fcimb.2020.00447. ISSN 2235-2988. PMC 7525035. PMID 33042855.
^ Jump up to:a b c d e f Delgado, Gabriela; Souza, Valeria; Morales, Rosario; Cerritos, René; González-González, Andrea; Méndez, José Luis; Vázquez, Virginia; Cravioto, Alejandro (2013). Webber, Mark Alexander (ed.). "Genetic Characterization of Atypical Citrobacter freundii". PLOS ONE. 8 (9): e74120. Bibcode:2013PLoSO...874120D. doi:10.1371/journal.pone.0074120. ISSN 1932-6203. PMC 3771896. PMID 24069274.
^ Jump up to:a b c Wertz, J. E.; Goldstone, C.; Gordon, D. M.; Riley, M. A. (2003). "A molecular phylogeny of enteric bacteria and implications for a bacterial species concept". Journal of Evolutionary Biology. 16 (6): 1236–1248. doi:10.1046/j.1420-9101.2003.00612.x. ISSN 1010-061X. PMID 14640415. S2CID 5755222.
^ Jump up to:a b c d Barlow, Miriam; Hall, Barry G (2003). "Experimental Prediction of the Evolution of Cefepime Resistance From the CMY-2 AmpC β-Lactamase". Genetics. 164 (1): 23–29. doi:10.1093/genetics/164.1.23. ISSN 1943-2631. PMC 1462546. PMID 12750318.
^ Pang, Allan; Warren, Martin J.; Pickersgill, Richard W. (2011). "Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe–4S cluster-binding site". Acta Crystallographica Section D: Biological Crystallography. 67 (2): 91–96. doi:10.1107/S0907444910050201. ISSN 0907-4449. PMID 21245529.
^ Jump up to:a b Snihur, H.; Petrenko, S.; Kot, T.; Shevchenko, O.; Polischuk, V. (2018). "Widespread Viral Diseases Endangering Cereal Crops in Ukraine". Mikrobiolohichnyi Zhurnal. 80 (3): 103–116. doi:10.15407/microbiolj80.03.103. ISSN 1028-0987. S2CID 134413047.
^ Jump up to:a b c d Tang, Jie; Lei, Dan; Wu, Min; Hu, Qiong; Zhang, Qing (2020). "Biodegradation and metabolic pathway of fenvalerate by Citrobacter freundii CD-9". AMB Express. 10 (1): 194. doi:10.1186/s13568-020-01128-x. ISSN 2191-0855. PMC 7599292. PMID 33125615.
^ Jump up to:a b c d e f g Anderson, Mark T.; Mitchell, Lindsay A.; Zhao, Lili; Mobley, Harry L. T. (2018). "Citrobacter freundii fitness during bloodstream infection". Scientific Reports. 8 (1): 11792. Bibcode:2018NatSR...811792A. doi:10.1038/s41598-018-30196-0. ISSN 2045-2322. PMC 6081441. PMID 30087402.
^ Schloissnig, Siegfried; Arumugam, Manimozhiyan; Sunagawa, Shinichi; Mitreva, Makedonka; Tap, Julien; Zhu, Ana; Waller, Alison; Mende, Daniel R.; Kultima, Jens Roat; Martin, John; Kota, Karthik (2013). "Genomic variation landscape of the human gut microbiome". Nature. 493 (7430): 45–50. Bibcode:2013Natur.493...45S. doi:10.1038/nature11711. ISSN 0028-0836. PMC 3536929. PMID 23222524.
^ Morowitz, M. J.; Denef, V. J.; Costello, E. K.; Thomas, B. C.; Poroyko, V.; Relman, D. A.; Banfield, J. F. (2011). "Strain-resolved community genomic analysis of gut microbial colonization in a premature infant". Proceedings of the National Academy of Sciences. 108 (3): 1128–1133. Bibcode:2011PNAS..108.1128M. doi:10.1073/pnas.1010992108. ISSN 0027-8424. PMC 3024690. PMID 21191099.
^ Whalen, Jason G. (2007). "Spontaneous Citrobacter freundii Infection in an Immunocompetent Patient". Archives of Dermatology. 143 (1): 124–125. doi:10.1001/archderm.143.1.124. ISSN 0003-987X. PMID 17224563.
