一、菌种简介
1、菌种名称：乳酸片球菌 Pediococcus acidilactici
2、菌种编号：TS722766
3、生物危害程度：四类
二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。
三、培养条件
1、培养基： MRS培养基
2、培养基成分：酪蛋白胨 10.0g，牛肉膏 10.0g，酵母粉 5.0g，葡萄糖 5.0g，乙酸钠 5.0g，柠檬酸二铵 2.0g，Tween 80 1.0g， K2HPO4 2.0g，MgSO4.7H2O 0.2g，MnSO4.H2O 0.05g，CaCO3 20.0g，琼脂 15.0g，蒸馏水 1.0L，pH6.8。推荐使用商品化 成品培养基。
3、需氧类型：好氧
4、培养温度：30
四、注意事项
1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。
2、试管斜面菌种请尽快转接，不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
References[edit]
^ Klaenhammer T.R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12 :39S-85S
^ Barros R.R., Carvalho G.S., Peralta J.M., Facklam R.R., Teixeira L.M. 2001. Phenotypic and genotypic characterization of Pediococcus strains isolated from human clinical sources. J. Clin Microbiol. April;39(4): 1241–1246.
^ Jump up to:a b Lin J.J. 2006. Probiotics as alternative Biomedicines for pets with digestive disorders
^ Daeschel M.A., Klaenhaemmer T.R. 1985. Association of a 13.6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl. Environ. Microbiol. 50: 1538S-1541S
^ Zhu, Liyan; Zeng, Jianwei; Wang, Chang; Wang, Jiawei (2022-02-08). "Structural Basis of Pore Formation in the Mannose Phosphotransferase System by Pediocin PA-1". Applied and Environmental Microbiology. 88 (3): e0199221. doi:10.1128/AEM.01992-21. ISSN 1098-5336. PMC 8824269. PMID 34851716.
^ Dalloul R.A., Lillehoj H.S, Lee J.S., Lee S.H., Chung K.S. 2006. Immunopotentiating effect of a Fomitella fraxinea – derived lectin on chicken immunity and resistance to coccidiosis. Poult. Sci. 85: 446S-451S
^ Ennahar S., Deschamps N. 2000. Anti-Listeria effect of enoterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins from lactic acid bacteria. J.Appl. Microbiol. 88:449S-457S
^ Lee SH, Lillehoj HS, Dalloul RA, Park DW, Hong YH, and Lin JJ. 2007. Influence of Pediococcus –based probiotic on coccidiosis in broiler chickens. Poultry Sci. 86:63-66
^ Jha, Rajesh; Das, Razib; Oak, Sophia; Mishra, Pravin (2020). "Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review". Animals. 10 (10): 1863. doi:10.3390/ani10101863. PMC 7602066. PMID 33066185.
^ Lee SH, Lillehoj HS, Park DW, Hong YH, and Lin JJ. 2007. Effects of Pediococcus –and Saccharomyces -based probiotic (MitoMax) on coccidiosis in broiler chickens. Comparative Immuno Microbiol & Infectious disease. 30:261-268
^ Jump up to:a b Lin J.J. 2006. Probiotics as alternative Biomedicines for pets with digestive disorders. Proceeding of 8th Annual meeting of JBVP. 3-288-292.
^ Jump up to:a b Mizutani W., Yamasaki R, Lin, JJ, Kuki M, and Kato G. 2007. Pediococcus-an [sic] unique probiotics we use as a novel GI supplement. Annual Meeting of JBVP. 3-269-3-272
