一、菌种简介

  1、菌种名称：单核增生李斯特氏菌 Listeria monocytogenes 

  2、菌种编号：TS715893 

  3、生物危害程度：三类 

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件

  1、培养基： 脑心琼脂 

  2、培养基成分：心提取物 5.0g，脑提取物 12.5g，月示 胨10.0g，葡萄糖 2.0g，NaCl 5.0g，Na2HPO4 2.5g，琼脂15.0g，蒸馏 水1.0L，pH7.4。推荐使用商品化成品培养基。

  3、需氧类型：好氧 

  4、培养温度：37 

四、注意事项 

  1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。

  2、试管斜面菌种请尽快转接，不建议长期存放。 

  3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。

 4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

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