发酵乳杆菌 Lactobacillus fermentum

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一、菌种简介

  1、菌种名称:发酵乳杆菌 Lactobacillus fermentum

  2、菌种编号:TS714524

  3、生物危害程度:四类

二、储存条件 冻干菌种和试管斜面请置于2℃~8℃冷藏。

三、培养条件 

  1、培养基: MRS培养基 

  2、培养基成分:酪蛋白胨 10.0g,牛肉膏 10.0g,酵母粉 5.0g,葡萄糖 5.0g,乙酸钠 5.0g,柠檬酸二铵 2.0g,Tween 80 1.0g, K2HPO4 2.0g,MgSO4.7H2O 0.2g,MnSO4.H2O 0.05g,CaCO3 20.0g,琼脂 15.0g,蒸馏水 1.0L,pH6.8。推荐使用商品化 成品培养基。

  3、需氧类型:好氧 

  4、培养温度:37 

四、注意事项 

  1、菌种常规培养时间:细菌1-2天,酵母3天,霉菌5-7天,大型真菌 7-10天。

  2、试管斜面菌种请尽快转接,不建议长期存放。 

  3、初次使用时请按照本说明书推荐条件进行复活培养,如使用其它类型培养基或培养条件造成菌种不活等损失,泰斯拓生物科技不负责任。

  4、使用者应保证菌种的安全存储和操作,带菌废弃物应高压灭菌处理后丢弃。

References[edit]

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  3. ^ Online Medical Dictionary - Lactobacillus Fermentum

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  13. Jump up to:a b Klein, Günter (February 2011). "Antibiotic Resistance and Molecular Characterization of Probiotic and Clinical Lactobacillus Strains in Relation to Safety Aspects of Probiotics". Foodborne Pathogens and Disease8 (2): 267–281. doi:10.1089/fpd.2010.0672PMID 21034236.

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