一、菌种简介

  1、菌种名称：头葡萄球菌 Staphylococcus capitis 

  2、菌种编号：TS766854 

  3、生物危害程度：四类 

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件 

  1、培养基： 营养肉汁琼脂 

  2、培养基成分：蛋白胨 5.0g，牛肉浸取物 3.0g，NaCl 5.0g，琼脂 15.0g，蒸馏水 1.0L，pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4∙H2O，则有利于产生芽孢。推荐使用商品化成品培养基。

  3、需氧类型：好氧

  4、培养温度：37 

四、注意事项

  1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。

  2、试管斜面菌种请尽快转接，不建议长期存放。 

  3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。

  4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

References[edit]

1. ^ Kloos, W. E.; Schleifer, K. H. (1975). "Isolation and Characterization of Staphylococci from Human Skin II. Descriptions of Four New Species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans". International Journal of Systematic Bacteriology. 25 (1): 62–79. doi:10.1099/00207713-25-1-62. ISSN 0020-7713.

2. ^ Van Der Zwet WC, Debets-Ossenkopp YJ, Reinders E, et al. (July 2002). "Nosocomial spread of a Staphylococcus capitis strain with heteroresistance to vancomycin in a neonatal intensive care unit". J. Clin. Microbiol. 40 (7): 2520–5. doi:10.1128/JCM.40.7.2520-2525.2002. PMC 120592. PMID 12089273.

3. ^ D'mello D, Daley AJ, Rahman MS, et al. (September 2008). "Vancomycin heteroresistance in bloodstream isolates of Staphylococcus capitis". J. Clin. Microbiol. 46 (9): 3124–6. doi:10.1128/JCM.00592-08. PMC 2546727. PMID 18596138.

4. ^ Iwase T, Seki K, Shinji H, Mizunoe Y, Masuda S (October 2007). "Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri". J. Med. Microbiol. 56 (Pt 10): 1346–9. doi:10.1099/jmm.0.47235-0. PMID 17893172.

5. ^ Kloos WE PMC 358308, Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev. 1994 Jan; 7(1): 117–140

6. ^ Tevell, S.; Hellmark, B.; Nilsdotter-Augustinsson, Å.; Söderquist, B. (2017). "Staphylococcus capitis isolated from prosthetic joint infections". European Journal of Clinical Microbiology & Infectious Diseases. 36 (1): 115–122. doi:10.1007/s10096-016-2777-7. PMC 5203848. PMID 27680718.

7. ^ Kloos, Wesley E. (1981), Maibach, Howard I.; Aly, Raza (eds.), "The Identification of Staphylococcus and Micrococcus Species Isolated from Human Skin", Skin Microbiology, New York, NY: Springer US, pp. 3–12, doi:10.1007/978-1-4612-5868-1_1, ISBN 978-1-4612-5870-4, retrieved 2021-12-13

8. ^ Otto, Michael (April 2010). "Staphylococcus colonization of the skin and antimicrobial peptides". Expert Review of Dermatology. 5 (2): 183–195. doi:10.1586/edm.10.6. ISSN 1746-9872. PMC 2867359. PMID 20473345.

9. ^ Jump up to:^a b Pizauro, Lucas José Luduverio; de Almeida, Camila Chioda; Silva, Saura Rodrigues; MacInnes, Janet I.; Kropinski, Andrew M.; Zafalon, Luiz Francisco; de Avila, Fernando Antônio; de Mello Varani, Alessandro (December 2021). "Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes". Scientific Reports. 11 (1): 17392. Bibcode:2021NatSR..1117392P. doi:10.1038/s41598-021-96842-2. ISSN 2045-2322. PMC 8405628. PMID 34462461.

10. ^ DEVRIESE, L.A.; SCHLEIFER, K.H.; ADEGOKE, G.O. (January 1985). "Identification of coagulase-negative staphylococci from farm animals". Journal of Applied Bacteriology. 58 (1): 45–55. doi:10.1111/j.1365-2672.1985.tb01428.x. ISSN 0021-8847. PMID 3980296.

11. ^ Jump up to:^a b c d Cameron, David R.; Jiang, Jhih-Hang; Hassan, Karl A.; Elbourne, Liam D. H.; Tuck, Kellie L.; Paulsen, Ian T.; Peleg, Anton Y. (2015-09-23). "Insights on virulence from the complete genome of Staphylococcus capitis". Frontiers in Microbiology. 6: 980. doi:10.3389/fmicb.2015.00980. ISSN 1664-302X. PMC 4585213. PMID 26441910.

12. ^ Cui, Bintao; Smooker, Peter M.; Rouch, Duncan A.; Daley, Andrew J.; Deighton, Margaret A. (January 2013). "Differences between Two Clinical Staphylococcus capitis Subspecies as Revealed by Biofilm, Antibiotic Resistance, and Pulsed-Field Gel Electrophoresis Profiling". Journal of Clinical Microbiology. 51 (1): 9–14. doi:10.1128/JCM.05124-11. ISSN 0095-1137. PMC 3536240. PMID 23052315.

13. ^ Tevell, S.; Hellmark, B.; Nilsdotter-Augustinsson, Å.; Söderquist, B. (January 2017). "Staphylococcus capitis isolated from prosthetic joint infections". European Journal of Clinical Microbiology & Infectious Diseases. 36 (1): 115–122. doi:10.1007/s10096-016-2777-7. ISSN 0934-9723. PMC 5203848. PMID 27680718.

14. ^ Sandoe, J A T; Kerr, K G; Reynolds, G W; Jain, S (1999-09-01). "Staphylococcus capitis endocarditis: two cases and review of the literature". Heart. 82 (3): e1. doi:10.1136/hrt.82.3.e1. ISSN 1355-6037. PMC 1729175. PMID 10455099.

15. ^ Butin, M.; Rasigade, J.-P.; Subtil, F.; Martins-Simões, P.; Pralong, C.; Freydière, A.-M.; Vandenesch, F.; Tigaud, S.; Picaud, J.-C.; Laurent, F. (November 2017). "Vancomycin treatment is a risk factor for vancomycin-nonsusceptible Staphylococcus capitis sepsis in preterm neonates". Clinical Microbiology and Infection. 23 (11): 839–844. doi:10.1016/j.cmi.2017.03.022. PMID 28373147