1、菌株名称：Pseudomonas chlororaphis (Guignard and Sauvageau 1894) Bergey et al. 绿针假单胞菌
2、菌株编号：TS274834
3、其他保藏编号：NBRC3521=ATCC13985
4、具体用途： 测序含铜亚硝酸盐还原酶基因
冻干菌种和试管斜面请置于2-8℃冷藏。
1、培养基：Nutrient Agar (营养肉汁琼脂)
2、培养基配方：Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g NaCl 5g Agar (琼脂) 15g Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2 [Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added . It is favorable to promote spore formation
3、培养温度： 30℃
1、冻干首次活化，干粉要全部用完，不能保留，用0.1-0.2ml的培养液或者无菌水溶解，接种在2支斜面上，因冻干菌种处在休眠状态，请勿接种多支斜面或平板，以免因接种量不足而导致复苏不成功。
2、试管斜面菌种请尽快转接，不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
Pseudomonas chlororaphis is a bacterium used as a soil inoculant in agriculture and horticulture. It can act as a biocontrol agent against certain fungal plant pathogens via production of phenazine-type antibiotics. Based on 16S rRNA analysis, similar species have been placed in its group.
A comparative genomic and phylogenomic study in 2020, analyzed 494 complete genomes from the entire Pseudomonas genus, with 43 of them being P. chlororaphis strains.[3] In this study, the P. chlororaphis species was determined, based on its monophyly and criterion of Average Nucleotide Identity. This species lies within the wider P. fluorescens species complex, as determined by. The protein count and GC content of the strains of this species ranged between 5599–6401 (average: 6076) and between 61.9–64% (average: 62.8%), respectively.In addition, the 43 P. chlororaphis proteomes contained 3587 core proteins (shared among all strains of the species), with 11 core proteins being specific for that group and thus absent in all other strains of the Pseudomonas genus. Two of these 11 group-specific core proteins are a holin family bacteriocin and a mitomycin-like biosynthetic protein and they may confer a competitive advantage against other root-colonizers.
Pseudomonas chlororaphis lends its name to a subgroup within the genus Pseudomonas. The other members of the P. chlororaphis subgroup are P. aurantiaca, P. aureofaciens, P. fragi, P. lundensis, and P. taetrolens.[2]
