1、菌株名称：雷氏普罗威登斯菌 Providencia rettgeri
3、生物危害程度：四类
冻干菌种和斜面试管请置于2-8℃冷藏。
1、脑心浸液（BHIA）：牛脑浸粉 4.0g，牛心浸粉 4.0g，蛋白胨 5.0g，酪蛋白胨16.0g，NaCl 5.0g，葡萄糖 2.0g，磷酸氢二钠 2.5g，pH7.4 ± 0.2。121℃，15min。
2、需氧类型：好氧
3、培养温度：37℃,24h
1、菌种常规培养时间：细菌24-48h，酵母72h，霉菌5-7d，大型真菌7-10d。
2、试管斜面请尽快转接，存放时间不得超过3个月。
3、初次使用请按照本说明书推荐条件进行活化，如使用其它类型培养基或培养条件造成菌种失活等损失，泰斯拓生物不负责任。
4、带菌废弃物应高压灭菌处理后丢弃。
Providencia rettgeri (commonly P. rettgeri), is a Gram negative bacterium that is commonly found in both water and land environments. P. rettgeri is in the genus Providencia, along with Providencia stuartii, Providencia alcalifaciens, and Providencia rustigianii. P. rettgeri can be incubated at 37 °C in nutrient agar or nutrient broth. It was first discovered in 1904 after a waterfowl epidemic. Strains of the species have also been isolated from nematodes of the genus Heterorhabditis. Providencia rettgeri also found in marine environment.
S.I. Paul et al. (2021) isolated, characterized and identified salt tolerant Providencia rettgeri from marine sponge (Niphates erecta) of the Saint Martin's Island Area of the Bay of Bengal, Bangladesh. Colony, morphological, physiological, and biochemical characteristics of Providencia rettgeri are shown in the Table below.
P. rettgeri can be identified by its motility and its ability to produce acid from mannitol. It does not produce gas from glucose and does not ferment lactose. It also does not produce hydrogen sulfide or acid from xylose.
Providencia rettgeri can cause a number of opportunistic infections in humans and can be found in the human gut. It is a major cause of traveller's diarrhea. Strains of P. rettgeri have also been found to cause urinary tract infections and eye infections.
Providencia rettgeri is a natural pathogen of Drosophila fruit flies. Susceptibility to P. rettgeri is strongly tied to an allele of the antimicrobial peptide gene Diptericin.The fly's defence against P. rettgeri seems to rely almost exclusively on Diptericin, as deletion of Diptericin leads to complete mortality. Meanwhile, deletion of multiple other antimicrobial peptides has no effect on P. rettgeri virulence. Yet defence against the related Providencia burhodogranariea is determined by multiple antimicrobial peptides beyond just Diptericin.
