1、菌种名称:托拉斯假单胞菌 Pseudomonas tolaasii
2、菌种编号:TS379407
3、生物危害程度:四类
冻干菌种和斜面试管请置于2-8℃冷藏。
1、营养肉汁琼脂:牛肉膏 3.0g,蛋白胨 10.0g,NaCl 5.0g,琼脂 15.0g,蒸馏水 1.0L,pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4·H2O,则有利于产生芽孢。pH7.0。
2、需氧类型:好氧
3、培养温度:30℃,48h
1、菌种常规培养时间:细菌1-2天,酵母3天,霉菌5-7天,大型真菌7-10天。
2、试管斜面种请尽快转接,不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养,如使用其它类型培养基或培养条件造成菌种不活等损失,泰斯拓生物不负责任。
4、使用者应保证菌种的安全储存和操作,带菌废弃物应高压灭菌处理后丢弃。
Pseudomonas tolaasii is a species of Gram-negative soil bacteria that is the causal agent of bacterial blotch on cultivated mushrooms (Agaricus bisporus).It is known to produce a toxin, called tolaasin, which is responsible for the brown blotches associated with the disease. It also demonstrates hemolytic activity, causing lysis of erythrocytes. Based on 16S rRNA analysis, P. tolaasii has been placed in the P. fluorescens group.