一、菌种简介 

1、菌种名称：施氏假单胞菌Pseudomonas stutzeri

2、菌种编号：TS274549

3、其他中心编号：ATCC17588

3、生物危害程度：

4、模式菌株：模式菌株、分类研究，减少嘧啶碱类

二、储存条件 

冻干菌种应在2~8℃保存;斜面菌种放15-20度保存,15-20天转接一次。

三、培养条件 

1、培养基编号：

·营养琼脂培养基(NA)

·胰蛋白胨大豆琼脂培养基(TSA)

·LB培养基  

2、培养时间：18-24小时

3、培养温度：30℃

四、注意事项 

·冻干首次活化,干粉要全部用完,不能保留,用0.1-0.2m1的培养液或者无菌水溶解,接种在2支斜面上,因冻干菌种处于休眠状态,请勿接种多支斜面或平板,以免因接种量不足而导致复苏不成功。

·复苏后,微生物菌种应保藏于建议的温度、清洁和干燥的地方,室温放置时间过长会导致菌种衰退;

·菌种操作应在无菌条件下进行; 转种完毕,废弃物应经灭菌再做丢弃处理,以免污染周围环境。

·冻干菌复苏后,应根据菌种状况及时转接传代;

Pseudomonas stutzeri is a Gram-negative soil bacterium that is motile, has a single polar flagellum, and is classified as bacillus, or rod-shaped. While this bacterium was first isolated from human spinal fluid, it has since been found in many different environments due to its various characteristics and metabolic capabilities. P. stutzeri is an opportunistic pathogen in clinical settings, although infections are rare. Based on 16S rRNA analysis, this bacterium has been placed in the P. stutzeri group, to which it lends its name.

Pseudomonas stutzeri is most easily differentiated from the other Pseudomonas spp. in that it does not produce fluorescent pigments. P. mendocina, P. alcaligenes, P. pseudoalcaligenes, and P. balearica are classified within the same branch of pseudomonads as P. stutzeri based on 16S rRNA sequences and other phylogenetic markers.[6] Of this group, P. stutzeri is most closely related to P. balearica and they can be differentiated not only by the 16S rRNA sequences, but also by the ability of P. stutzeri to grow above 42 °C. P. stutzeri has been isolated in many different locations, and since each strain is a little different based on where it was isolated, the Pseudomonas stutzerigroup contains many genomovars.[6] This means that the many strains of P. stutzeri can be considered genospecies, which are organisms that can only be differentiated based on their nucleic acid composition.

Burri and Stutzer first described Pseudomonas stutzeri in 1895 and named the bacterium Bacillus denitrificans II. Seven years later in 1902, Itersonion developed an enrichment culture for P. stutzeri, which was later described by van Niel and Allen in 1952. The enrichment medium is a mineral medium with 2% nitrate and tartrate (or malate, succinate, malonate, citrate, ethanol, or acetate) grown under anaerobic conditions at 37 °C.The organism has been isolated from a wide variety of places such as human spinal fluid, straw, manure, soil, and canal water.