一、菌种简介
1、菌种名称:肠沙门氏菌肠亚种鼠伤寒血清型 Salmonella enterica subsp. enterica serotype Typhimurium
2、菌种编号:TS945686
3、生物危害程度:三类
二、储存条件 冻干菌种和试管斜面请置于2℃~8℃冷藏。
三、培养条件
1、培养基: 营养肉汁琼脂
2、培养基成分:蛋白胨 5.0g,牛肉浸取物 3.0g,NaCl 5.0g,琼脂 15.0g,蒸馏水 1.0L,pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4∙H2O,则有利于产生芽孢。推荐使用商品化成品培养基。
3、需氧类型:好氧
4、培养温度:36
四、注意事项
1、菌种常规培养时间:细菌1-2天,酵母3天,霉菌5-7天,大型真菌 7-10天。
2、试管斜面菌种请尽快转接,不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养,如使用其它类型培养基或培养条件造成菌种不活等损失,泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作,带菌废弃物应高压灭菌处理后丢弃。
Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.
Judicial Commission of the Internat. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int J Syst Evol Microbiol 55: 519-520, 2005. PubMed: 15653929
Tindall BJ, et al. Nomenclature and taxonomy of the genus Salmonella. Int J Syst Evol Microbiol 55: 521-524, 2005. PubMed: 15653930
Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005
Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.
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