一、菌种简介 

 1、菌种名称：巨大芽胞杆菌 Bacillus megaterium

 2、菌种编号：TS964568

 3、生物危害程度：四类 

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件 

 1、培养基： 营养肉汁琼脂 

 2、培养基成分：蛋白胨 5.0g，牛肉浸取物 3.0g，NaCl 5.0g，琼脂 15.0g，蒸馏水 1.0L，pH7.0。[注] 培养芽孢杆菌时加入5mg MnSO4∙H2O，则有利于产生芽孢。推荐使用商品化成品培养基。

 3、需氧类型：好氧 

 4、培养温度：30 

四、注意事项 

 1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。

 2、试管斜面菌种请尽快转接，不建议长期存放。

 3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。

 4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

References[edit]

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3. ^ Jump up to:^a b Bunk, B. et al. A short story about a big magic bug. Bioengineered Bugs 1:85–91 (2010)

4. ^ Gupta, Radhey S.; Patel, Sudip; Saini, Navneet; Chen, Shu (2020-11-01). "Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species". International Journal of Systematic and Evolutionary Microbiology. 70 (11): 5753–5798. doi:10.1099/ijsem.0.004475. ISSN 1466-5026. PMID 33112222.

5. ^ Pueyo, Manuel Troyano; Bloch, Carlos; Carmona-Ribeiro, Ana Maria; di Mascio, Paolo (2008-10-29). "Lipopeptides Produced by a Soil Bacillus Megaterium Strain". Microbial Ecology. 57 (2): 367–378. doi:10.1007/s00248-008-9464-x. ISSN 1432-184X. PMID 18958512. S2CID 266751.

6. ^ Shimizu, K., Nakamura, H. & Ashiuchi, M. Salt-Inducible Bionylon Polymer from Bacillus Megaterium. Appl. Environ. Microbiol. 73:2378–2379 (2007)

7. ^ Khan, J. A. Biodegradation of Azo Dye by Moderately Halotolerant Bacillus megaterium and Study of Enzyme Azoreductase Involved in Degradation. Advanced Biotech 10:21–27 (2011)

8. ^ Shwed P.S. et al. Complete Genome Sequences of Priestia megaterium type and clinical strains feature complex plasmid arrays. Microbiol. Resource Announcements 10(27):e00403-21(2021)

9. ^ Dib, E. G. et al. Nonhemolytic, Nonmotile Gram-Positive Rods Indicative of Bacillus anthracis. Emerg Infect Dis. 9:1013–1015 (2003)

10. ^ Mohammad, Salma Malihah; Mahmud-Ab-Rashid, Nor-Khaizura; Zawawi, Norhasnida (2020-08-25). "Probiotic properties of bacteria isolated from bee bread of stingless bee Heterotrigona itama". Journal of Apicultural Research. 60: 172–187. doi:10.1080/00218839.2020.1801152. ISSN 0021-8839. S2CID 225208290.

11. ^ Claus, D. Anreicherung und Direktisolierung aerober sporenbildender Bakterien. In Schlegel, pp 337–362 (1965)

12. ^ DE BARY (A.): Vergleichende Morphologie und Biologie der Pilze, Mycetozoen und Bacterien. Wilhelm Engelmann, Leipzig, 1884.

13. ^ Jump up to:^a b c Buchanan, R. E.; Breed, R. S.; St. John-Brooks, R. (1951). "Opinion 1. The Correct Spelling of the Specific Epithet in the Species Name Bacillus Megaterium De Bary 1884: Approved by the Judicial Commission of the International Committee on Bacteriological Nomenclature". International Bulletin of Bacteriological Nomenclature and Taxonomy. 1: 35–36. doi:10.1099/0096266X-1-1-35.

14. ^ Nahid, E.-A. Phenotypic and Genetic Variability Among Three Bacillus Megatherium Isolates. I. In Viro Evoluation of Tri-Calcium Phosphate Solubilizing Potential and Growth Pattern. J Am Sc 6:111–115 (2010)

15. ^ Du, X. et al. Correlation of bacterial diversity in rot Chinese cabbage with the habitat. Wei Sheng Wu Xue Bao 51:1639-45 (2011)

16. ^ Lapage, S.; Sneath, P.; Lessel, E.; Skerman, V.; Seeliger, H.; Clark, W. (1992). International Code of Nomenclature of Bacteria: Bacteriological Code, 1990 Revision. Washington, D.C.: ASM Press. PMID 21089234.

17. ^ μέγας

18. ^ θηρίον

19. ^ Rippel, Arch Mikrobiol. 11, 470, 1940

20. ^ τέρας

21. ^ B. megaterium entry in LPSN; Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet". International Journal of Systematic and Evolutionary Microbiology. 47 (2): 590–2. doi:10.1099/00207713-47-2-590. PMID 9103655.