一、菌种简介 

 1、菌种名称：布氏乳杆菌 Lactobacillus buchneri

 2、菌种编号：TS957879

 3、生物危害程度：四类 

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件 

 1、培养基： MRS培养基 

 2、培养基成分：酪蛋白胨 10.0g，牛肉膏 10.0g，酵母粉 5.0g，葡萄糖 5.0g，乙酸钠 5.0g，柠檬酸二铵 2.0g，Tween 80 1.0g， K2HPO4 2.0g，MgSO4.7H2O 0.2g，MnSO4.H2O 0.05g，CaCO3 20.0g，琼脂 15.0g，蒸馏水 1.0L，pH6.8。推荐使用商品化 成品培养基。

 3、需氧类型：好氧 

 4、培养温度：30 

四、注意事项 

 1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。 

 2、试管斜面菌种请尽快转接，不建议长期存放。 

 3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。

 4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

References[edit]

1. ^ FRED, E.B., PETERSON,W.H., AND Anderson, J. A., J.Biol. Chem., 48, 385 (1921).

2. ^ Jump up to:^a b Ranjit, N.K.; Kung, L. Jr. (2000). "The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage". Journal of Dairy Science. 88 (3): 526–35. doi:10.3168/jds.S0022-0302(00)74912-5. PMID 10750111.

3. ^ Sandor, G.K.B.; Low, D.E.; Judd, P.L.; Davidson, R.J. (1998). "Antimicrobial Treatment Options In the Management Of Odontogenic Infections". Journal (Canadian Dental Association). Canadian Dental Association. 64 (7): 508–514. PMID 9737082.

• Driehuis, F., W. J. W. H. Oude Elferink, and S. F. Spoelstra. 1999. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J. Appl. Microbiol. 87:583-594.

• Danner, H., Holzer, Mayrhuber, E., and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology 69(1) 562-567.

• Grazia, L., Suzzi, G., 1984. A survey of lactic acid bacteria in Italian silage. Journal of Applied Bacteriology 56:373-379.

• Knapp, J. 2012. Does Converting Simple Sugars to Lactic Acid Reduce the Energy Available in Silages? Feedstuffs:

• Kung Jr, L. 2010. Aerobic stability of silage. Proceeding 2010 California Alfalfa & Forage Symposium and Corn/Cereal Silage Conference. University of California.

• Ranjit, N.K. and Kung, Jr, L. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. Journal of Dairy Science 83:526-535.

• Schmidt, R. J., Hu, W., Mills, J. A., and Kung, Jr, L. 2009. The development of lactic acid bacteria and Lactobacillus buchneri and their effects on the fermentation of alfalfa silage. Journal of Dairy Science 92:5005-5010.

• Oude Elferink, S., Krooneman, J., Gottschal, J., Spoelstra, S., Faber, F. and Drlehuls, F. 2001 Anaerobic conversion of lactic acid to acetic acid and 1,2 propanediol by Lactobacillus buchneri. Applied and Environmental Microbiology. 67:125-132

• Thomas, K.C., Hynes, S. H., and Ingledew, W.M. 2002. Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids. Applied and Environmental Microbiology 68(4) 1616-1623.

• Johns Hopkins POC IT Center: Lactobacillus

• MayoClinic.com: Reye's Syndrome

• The Merck Manual Online Medical Library: Intravenous Fluid Resuscitation