一、菌种简介
1、菌种名称:杰丁毕赤酵母 Pichia jadinii
2、菌种编号:TS898282
3、生物危害程度:四类
二、储存条件 冻干菌种和试管斜面请置于2℃~8℃冷藏。
三、培养条件
1、培养基: 5 °Bé麦芽汁琼脂
2、培养基成分:5°Bé麦芽汁 1.0L(或用MEB代替),琼脂 15.0g,自然pH。推荐使用商品化成品培养基。
3、需氧类型:好氧
4、培养温度:28
四、注意事项
1、菌种常规培养时间:细菌1-2天,酵母3天,霉菌5-7天,大型真菌 7-10天。
2、试管斜面菌种请尽快转接,不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养,如使用其它类型培养基或培养条件造成菌种不活等损失,泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作,带菌废弃物应高压灭菌处理后丢弃。
Buckholz RG. Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same. US Patent 5,268,273 dated Dec 7 1993
Vozza LA, et al. Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Bio-Technology 14: 77-81, 1996. PubMed: 9636316
Payne WE, et al. An inducible acid phosphatase from the yeast Pichia pastoris: characterization of the gene and its product. Gene 163: 19-26, 1995. PubMed: 7557473
Zhu A, et al. High-level expression and purification of coffee bean alpha- galactosidase produced in the yeast Pichia pastoris. Arch. Biochem. Biophys. 324: 65-70, 1995. PubMed: 7503561
Cregg JM, et al. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5: 3376-3385, 1985. PubMed: 3915774
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