一、菌种简介
1、菌种名称:红冬孢酵母 Rhodosporidium toruloides
2、菌种编号:TS891466
3、生物危害程度:四类
二、储存条件 冻干菌种和试管斜面请置于2℃~8℃冷藏。
三、培养条件
1、培养基: 5 °Bé麦芽汁琼脂
2、培养基成分:5°Bé麦芽汁 1.0L(或用MEB代替),琼脂 15.0g,自然pH。推荐使用商品化成品培养基。
3、需氧类型:好氧
4、培养温度:28
四、注意事项
1、菌种常规培养时间:细菌1-2天,酵母3天,霉菌5-7天,大型真菌 7-10天。
2、试管斜面菌种请尽快转接,不建议长期存放。
3、初次使用时请按照本说明书推荐条件进行复活培养,如使用其它类型培养基或培养条件造成菌种不活等损失,泰斯拓生物科技不负责任。
4、使用者应保证菌种的安全存储和操作,带菌废弃物应高压灭菌处理后丢弃。
Hu J, Ji L. Draft genome sequences of Rhodosporidium toruloides strains ATCC 10788 and ATCC 10657 with compatible mating types. Genome Announc 4: e00098-16, 2016. PubMed: 26966203
Liu Y, et al. Characterization of glyceraldehyde-3-phosphate dehydrogenase gene RtGPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides. Appl. Microbiol. Biotechnol. 97: 719-729, 2013. PubMed: 22722909
Hamamoto M, Nagahama T, Tamura M. Systematic study of basidiomycetous yeasts - evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium. FEMS Yeast Res. 2: 409-413, 2002. PubMed: 12702292
Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266
Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082
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