一、菌种简介 

  1、菌种名称：绿木霉 Trichoderma virens 

  2、菌种编号：TS779275 

  3、生物危害程度：四类 

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件

  1、培养基： 马铃薯、葡萄糖琼脂培养基(PDA) 

  2、培养基成分：马铃薯提取液 1.0L，葡萄糖 20.0g，琼脂 15.0g，pH自然。[注] 马铃薯提取液：取去皮马铃薯200g，切成小块， 加水1.0L煮沸30min，滤去马铃薯块，将滤液补足至1.0L。可以使用商品化成品培养基。

  3、需氧类型：好氧

  4、培养温度：24

四、注意事项 

  1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。

  2、试管斜面菌种请尽快转接，不建议长期存放。 

 3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。 

 4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

References[edit]

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6. ^ Jump up to:^a b Samuels, Gary J. (2006). "Trichoderma: Systematics, the Sexual State, and Ecology" (PDF). Phytopathology. 96 (2): 195–206. doi:10.1094/PHYTO-96-0195. ISSN 0031-949X. PMID 18943925.

7. ^ Yang, Chin (2007). SAMPLING AND ANALYSIS OF INDOOR MICROORGANISMS. Wiley. p. 259. ISBN 9780471730934.

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9. ^ Beyer, W.M.; Wuest, P.J.; Anderson, M.G. "Green mold of Mushrooms". Pennsylvania State University. Retrieved 2007-08-02. Pennsylvania State University extension bulletin

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11. ^ Li Destri Nicosia, M.G.; Mosca, S.; Mercurio, R.; Schena, L. (2015). "Dieback of Pinus nigra Seedlings Caused by a Strain of Trichoderma viride". Plant Disease. 99 (1): 44–49. doi:10.1094/PDIS-04-14-0433-RE. PMID 30699733.

12. ^ Reason Discovered for the Toxicity of Indoor Mould – ScienceDaily (Oct. 12, 2012) : https://www.sciencedaily.com/releases/2012/10/121012074655.htm

13. ^ Mikkola, Raimo; Andersson, Maria A.; Kredics, László; Grigoriev, Pavel A.; Sundell, Nina; Salkinoja-Salonen, Mirja S. (2012). "20-Residue and 11-residue peptaibols from the fungus Trichoderma longibrachiatum are synergistic in forming Na + /K + -permeable channels and adverse action towards mammalian cells". FEBS Journal. 279 (22): 4172–4190. doi:10.1111/febs.12010. PMID 22994321.

14. ^ “Trilongins” Offer Insight into Mold Toxicity Archived 2016-03-11 at the Wayback Machine Environmental health perspectives 2/2013.

15. ^ Dreyfuss, M; Härri, E; Hofmann, H; Kobel, H; Pache, W; Tscherter, H (1976). "Cyclosporin A and C: new metabolites from Trichoderma polysporum (Link ex Pers.) Rifai". European Journal of Applied Microbiology. 3: 125–133. doi:10.1007/bf00928431.

16. ^ Authors; Chong, FW; Chakravarthi, S; Nagaraja, HS; Thanikachalam, PM; Lee, N (Jun 2009). "Expression of transforming growth factor-beta and determination of apoptotic index in histopathological sections for assessment of the effects of Apigenin (4', 5', 7'- Trihydroxyflavone) on Cyclosporine A induced renal damage". Malays J Pathol. 31 (1): 35–43. PMID 19694312.

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