一、菌种简介

  1、菌种名称：产气荚膜梭菌 Clostridium perfringens 

  2、菌种编号：TS777864 

  3、生物危害程度：三类

二、储存条件 冻干菌种和试管斜面请置于2℃～8℃冷藏。

三、培养条件 

  1、培养基： MTS琼脂(胰酶消化碎肉半流体培养基，厌氧梭菌用)

  2、培养基成分：牛肉胰酶消化液 500.0ml，牛肉浸液 500.0ml，NaCl 5.0g，葡萄糖 5.0g，琼脂 1g，硫乙醇酸钠 0.5g，碎肉渣 10g，pH7.2～7.4。

  3、需氧类型：厌氧 

  4、培养温度：37

四、注意事项 

  1、菌种常规培养时间：细菌1-2天，酵母3天，霉菌5-7天，大型真菌 7-10天。

  2、试管斜面菌种请尽快转接，不建议长期存放。 

  3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物科技不负责任。

  4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。

References[edit]

1. ^ Ryan, Kenneth J.; Ray, C. George (2004). Sherris Medical Microbiology : an Introduction to Infectious Diseases (4th ed.). New York: McGraw-Hill. p. 310. ISBN 978-0-8385-8529-0.

2. ^ Kiu, R; Hall, L. J. (2018). "An update on the human and animal enteric pathogen Clostridium perfringens". Emerging Microbes & Infections. 7 (141): 141. doi:10.1038/s41426-018-0144-8. PMC 6079034. PMID 30082713.

3. ^ "BioNumber Details Page". BioNumbers.

4. ^ "Foodborne Illnesses and Germs". Centers for Disease Control and Prevention (CDC). 2018-02-16. Retrieved 18 February 2018.

5. ^ Jump up to:^a b Juckett, G; Bardwell, G; McClane, B; Brown, S (2008). "Microbiology of salt rising bread". The West Virginia Medical Journal. 104 (4): 26–7. PMID 18646681.

6. ^ Lexicon Orthopaedic Etymology. CRC Press. 1999. p. 128. ISBN 9789057025976.

7. ^ Jump up to:^a b c Kiu, Raymond; Caim, Shabhonam; Alexander, Sarah; Pachori, Purnima; Hall, Lindsay J. (2017). "Probing Genomic Aspects of the Multi-Host Pathogen Clostridium perfringens Reveals Significant Pangenome Diversity, and a Diverse Array of Virulence Factors". Frontiers in Microbiology. 8: 2485. doi:10.3389/fmicb.2017.02485. PMC 5733095. PMID 29312194.

8. ^ Liu, Hualan; McCord, Kristin D.; Howarth, Jonathon; David, Popham L.; Jensen, Roderick V.; Melville, Stephen B. (July 2014). "Hypermotility in Clostridium perfringens Strain SM101 Is Due to Spontaneous Mutations in Genes Linked to Cell Division". Journal of Bacteriology. 196 (13): 2405–2412. doi:10.1128/JB.01614-14. PMC 4054169. PMID 24748614.

9. ^ Kiu, Raymond; Caim, Shabhonam; Painset, Anais; Pickard, Derek; Swift, Craig; Dougan, Gordon; Mather, Alison E.; Amar, Corinne; Hall, Lindsay J. (25 September 2019). "Phylogenomic analysis of gastroenteritis-associated Clostridium perfringens in England and Wales over a 7-year period indicates distribution of clonal toxigenic strains in multiple outbreaks and extensive involvement of enterotoxin-encoding (CPE) plasmids". Microbial Genomics. 5 (10). doi:10.1099/mgen.0.000297. PMC 6861862. PMID 31553300.

10. ^ Labbe, R.G.; Juneja, V.K. (2017), "Clostridium perfringens", Foodborne Diseases, Elsevier, pp. 235–242, doi:10.1016/b978-0-12-385007-2.00010-3

11. ^ Warrell; et al. (2003). Oxford Textbook of Medicine (4th ed.). Oxford University Press. ISBN 978-0-19-262922-7.^{[page needed]}

12. ^ Murray; et al. (2009). Medical Microbiology (6th ed.). Mosby Elsevier. ISBN 978-0-323-05470-6.^{[page needed]}

13. ^ Jump up to:^a b Lentino, Joseph R. (2016-01-01). "Clostridial Necrotizing Enteritis". Merck Manuel. Merck Sharp & Dohme Corp. Retrieved 2016-09-27.

14. ^ Jump up to:^a b c "Clostridium perfringens". Center for Disease Control and Prevention. 2015-10-08. Retrieved 2016-09-27.

15. ^ "Gas gangrene: MedlinePlus Medical Encyclopedia".

16. ^ Rumah, Kareem Rashid; Linden, Jennifer; Fischetti, Vincent A.; Vartanian, Timothy; Esteban, Francisco J. (16 October 2013). "Isolation of Clostridium perfringens Type B in an Individual at First Clinical Presentation of Multiple Sclerosis Provides Clues for Environmental Triggers of the Disease". PLOS ONE. 8 (10): e76359. Bibcode:2013PLoSO...876359R. doi:10.1371/journal.pone.0076359. PMC 3797790. PMID 24146858.

17. ^ "Multiple sclerosis 'linked to food bug'". BBC. 29 January 2014. Retrieved 29 January 2014.

18. ^ Woerner, Amanda (29 January 2014). "Bacterial toxin may trigger multiple sclerosis, research finds". Fox News.

19. ^ Harpold, D. J.; Wasilauskas, B. L.; O'Connor, M. L. (1985). "Rapid identification of Clostridium species by high-pressure liquid chromatography" (PDF). Journal of Clinical Microbiology. 22 (6): 962–967. doi:10.1128/jcm.22.6.962-967.1985. PMC 271860. PMID 3905852.

20. ^ "Bad Bug Book (Second Edition)" (PDF). U.S. Food and Drug Administration. 2014-10-07. Retrieved 2016-09-26.

21. ^ ICMSF Microorganisms in Foods 5: Characteristics of Microbial Pathogens. London Blackie. 1996.

22. ^ Di Bella, Stefano; Antonello, Roberta Maria; Sanson, Gianfranco; Maraolo, Alberto Enrico; Giacobbe, Daniele Roberto; Sepulcri, Chiara; Ambretti, Simone; Aschbacher, Richard; Bartolini, Laura; Bernardo, Mariano; Bielli, Alessandra (June 2022). "Anaerobic bloodstream infections in Italy (ITANAEROBY): A 5-year retrospective nationwide survey". Anaerobe. 75: 102583. doi:10.1016/j.anaerobe.2022.102583.

23. ^ Jawetz Melnick & Adelbergs Medical Microbiology - 27E.

24. ^ Johnson, E. A., Summanen, P., & Finegold, S. M. (2007). Clostridium. In P. R. Murray (Ed.), Manual of Clinical Microbiology (9th ed., pp. 889-910). Washington, D.C.: ASM Press

25. ^ Songer, J. G. (2010). Clostridia as agents of zoonotic disease. Veterinary Microbiology, 140(3-4), 399-404

26. ^ Grass, Julian E.; Gould, L. Hannah; Mahon, Barbara E. (1 February 2013). "Epidemiology of foodborne disease outbreaks caused by Clostridium perfringens, United States, 1998-2010". Foodborne Pathog. Dis. 10 (2): 131–136. doi:10.1089/fpd.2012.1316. PMC 4595929. PMID 23379281.

27. ^ "Fatal Foodborne Clostridium perfringens Illness at a State Psychiatric Hospital — Louisiana, 2010". Centers for Disease Control and Prevention. Retrieved 16 November 2013.

28. ^ Mohn, Tanya (1 December 2011). "Passenger dies in-flight, family says airline to blame". Overhead Bin. MSNBC. Retrieved 2012-02-13.

29. ^ "Pub chef and manager jailed over Christmas dinner that left a diner dead". The Guardian. 23 January 2015. Retrieved 3 August 2015.

30. ^ "Woman's death likely caused by bacteria from Christmas supper". CBC. 12 December 2014.

31. ^ "Food poisoning death at Indiana restaurant kept secret for months". 13 WTHR Indianapolis. 2017-07-17. Retrieved 2017-07-18.

32. ^ (WTHR), Susan Batt. "Agave Azul Tippecanoe Co Food Poisoning Finding Summary". www.documentcloud.org. Retrieved 2017-07-18.

33. ^ "Bacteria that killed 3 at Antioch Thanksgiving dinner pinpointed". SFGate. Retrieved 2016-12-20.

34. ^ "Mother, son sue eatery for Thanksgiving dinner food poisoning - Food Safety News". 6 January 2017.

35. ^ "CDC releases test findings after hundreds sickened at Powell Chipotle - Columbus Dispatch". 16 August 2018.

36. ^ "Strain of food poisoning causes illness at North Carolina church barbecue". November 2018.

37.