LOCUS       Exported                4900 bp ds-DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..339
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      190..325
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             362..925
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /note="DHFR"
                     /translation="MVRPLNCIVAVSQDMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     intron          1082..1147
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             1277..1297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    1722..1856
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2709..2813
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2814..3674
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3845..4433
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4619..4759
                     /note="bom"
                     /note="basis of mobility region from pBR322"