LOCUS       Exported                8369 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  retrograde AAV helper vector, carrying rep and modified capsid gene.
ACCESSION   .
VERSION     .
KEYWORDS    rAAV2-retro helper
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo
            S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J,
            Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY
  TITLE     A Designer AAV Variant Permits Efficient Retrograde Access to 
            Projection Neurons.
  JOURNAL   Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021.
  PUBMED    27720486
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021."
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   44..146
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             4249..4260
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      5186..5699
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5273..5292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6181..6200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6300..6322
                     /label=pGEX 3
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6360..6378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6551..7411
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"