LOCUS Exported 8509 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8509)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8509
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(5843..5945)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(6471..7016)
/direction=LEFT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 7128..7156
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(7499..8215)
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/note="mgfp5"
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
LOCUS Exported 8509 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8509)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8509
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(5843..5945)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(6471..7016)
/direction=LEFT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 7128..7156
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(7499..8215)
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/note="mgfp5"
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
