LOCUS       Exported                5015 bp ds-DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5015)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        304..507
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5UTR           597..636
                     /note="HSV TK 5-UTR"
                     /note="5 untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          704..1276
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1473..1528
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(1865..2061)
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      1879..2014
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      complement(2299..2887)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3058..3918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3919..4023)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(4355..4810)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4955..4971
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4978..4996
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5015..303
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"