pBlueBacHis2 A (4863 bp), pBlueBacHis2 B (4853 bp), and pBlueBacHis2 C (4861 bp) are baculovirus transfer vectors that contain the Xpress N-terminal tag for expression and purification of recombinant fusion proteins. The pBlueBacHis2 vector is provided in three different versions for simplified cloning of your gene in-frame with the Xpress tag. The vectors contain sequences homologous to the lacZ gene and ORF1629 sequences in Bac-N-Blue linear DNA, allowing production of blue, recombinant plaques. Expression of your recombinant fusion protein is driven by the polyhedrin promoter.
| 载体基本信息 | |||
| 载体名称 | pBlueBacHis2 B | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 4853 bp | 载体类型 | Insect Cell Expression Vectors |
| 拷贝数 | High copy number | 启动子 | Polyhedrin |
| 克隆方法 | Enzyme digestion and ligation | 5引物 | polyhedrin forward |
| 载体标签 | His | ||
pBlueBacHis2 B 载体载体图谱
pBlueBacHis2 B 载体载体序列
LOCUS Exported 4853 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4853)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4853
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 4..94
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 110..127
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 131..163
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 167..190
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
misc_recomb 257..962
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
3UTR 345..717
/note="polyhedrin 3 UTR"
/note="3 UTR from the Autographa californica
nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
CDS complement(374..962)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
promoter 1538..1642
/gene="bla"
/note="AmpR promoter"
CDS 1643..2503
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2674..3262
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
