| 载体基本信息 |
| 载体名称 |
pKW08-Lx-Int |
载体抗性 |
Hygromycin |
| 载体长度 |
8535 bp |
载体类型 |
Bacterial Expression |
| 拷贝数 |
Low Copy |
克隆方法 |
Restriction Enzyme |
pKW08-Lx-Int 载体载体图谱
pKW08-Lx-Int 载体载体序列
LOCUS Exported 8535 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKW08-Lx-Int
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8535)
AUTHORS Williams KJ, Joyce G, Robertson BD
TITLE Improved mycobacterial tetracycline inducible vectors.
JOURNAL Plasmid. 2010 Apr 29. ():.
PUBMED 20434484
REFERENCE 2 (bases 1 to 8535)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid.
2010 Apr 29. ():."
FEATURES Location/Qualifiers
source 1..8535
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 14..41
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(2903..2919)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2904..2920)
/label=pBluescriptKS
/note="For pBluescript vector"
terminator 3693..3739
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin 7700..8288
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8189..8208
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8442..8459
/label=L4440
/note="L4440 vector, forward primer"
