LOCUS       Exported               10725 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  HPV52 L1 and L2 Bicistronic, too large to self-package.
ACCESSION   .
VERSION     .
KEYWORDS    p52sheLL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Kondo K, Ochi H, Matsumoto T, Yoshikawa H, Kanda T
  TITLE     Modification of human papillomavirus-like particle vaccine by 
            insertion of the cross-reactive L2-epitopes.
  JOURNAL   J Med Virol. 2008 May;80(5):841-6. doi: 10.1002/jmv.21124.
  PUBMED    18360909
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jmv"; 
            journalName: "J Med Virol"; date: "2008-05"; volume: "80"; issue: 
            "5"; pages: "841-6"
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3662..4250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3715..3735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4133..4144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4287..4304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4293..4517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4563..4991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4650..4669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4860..4881
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5000..5020)
                     /label=pBABE 3
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6949..7317
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     polyA_signal    7447..7568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7484..7503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7538..7557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7617..7633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7617..7633)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7630..7652)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7641..7657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7665..7695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7710..7731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7848..7865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8019..8604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8099..8118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8775..9635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9398..9417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9636..9740)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9815..9834)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10006..10385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10386..10589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10540..10560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10591..10609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10612..10630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10640..10664
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"