LOCUS       Exported                5708 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSMT3-M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(436..452)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(437..453)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      4672..5260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5161..5180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5414..5431
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5548..5569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5584..5614
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5622..5638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5627..5649
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5646..5662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5646..5662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"