LOCUS       Exported                6716 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  UAS-less reporter vector YIpMELalpha2, complete sequence.
ACCESSION   AY428062
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
  PUBMED    10773444
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt 
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 6716)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="UAS-less reporter vector YIpMELalpha2"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:248887"
     misc_feature    complement(31..1825)
                     /label=MEL1 orf
                     /note="MEL1 orf"
     regulatory      complement(1826..2478)
                     /regulatory_class="promoter"
                     /note="UAS deleted MEL1 promoter"
     primer_bind     complement(2488..2504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2777..3580)
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=URA3
                     /note="URA3p"
                     /protein_id="AAR04163.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            complement(2777..3580)
                     /gene="URA3"
                     /label=URA3
     promoter        complement(3581..3791)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        4595..4699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4700..5560
                     /codon_start=1
                     /gene="ampR"
                     /product="beta lactamase"
                     /label=ampR
                     /protein_id="AAR04164.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4700..5560
                     /gene="ampR"
                     /label=ampR
     rep_origin      5731..6319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6643..6673
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6681..6697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6705..5
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"