LOCUS       Exported                8655 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integrating expression vector YIpDCE1, complete sequence.
ACCESSION   AF039102
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Stotz A, Linder P.
  TITLE     The ADE2 gene from Saccharomyces cerevisiae: sequence and new 
            vectors
  JOURNAL   Gene 95 (1), 91-98 (1990)
  PUBMED    2253890
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     YIpDCE1 - an integrating plasmid for dual constitutive expression in
            yeast
  JOURNAL   Gene 212 (2), 197-202 (1998)
  PUBMED    9611262
REFERENCE   3  (bases 1 to 8655)
  AUTHORS   Stearman R, Dancis A, Klausner RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-1997) CBMB, NIH/NICHD, 9000 Rockville Pike, 
            Bethesda, MD 20892, USA
REFERENCE   4  (bases 1 to 8655)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Integrating expression vector YIpDCE1"
                     /host="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:75737"
     regulatory      306..1800
                     /regulatory_class="promoter"
                     /note="phosphoglycerate kinase"
     misc_feature    1801..1846
                     /note="polylinker; multiple cloning site 1"
     regulatory      1847..2223
                     /regulatory_class="terminator"
                     /note="phosphoglycerate kinase"
     primer_bind     complement(2263..2279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2287..2303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2311..2341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2356..2377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2442..2817
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             2818..4533
                     /codon_start=1
                     /gene="ADE2"
                     /product="phosphoribosylamino-imidazole-carboxylase"
                     /function="adenine biosynthesis"
                     /label=ADE2
                     /protein_id="AAC40224.1"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     gene            2818..4533
                     /gene="ADE2"
                     /label=ADE2
     regulatory      4808..6300
                     /regulatory_class="promoter"
                     /note="phosphoglycerate kinase"
     misc_feature    6301..6330
                     /note="polylinker; multiple cloning site 2"
     regulatory      6331..6707
                     /regulatory_class="terminator"
                     /note="phosphoglycerate kinase"
     rep_origin      complement(6836..7424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7595..8455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8456..8560)
                     /gene="bla"
                     /label=AmpR promoter