LOCUS       Exported               12840 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  VEEV replicon vector YFV-C3opt-NS2mut, complete sequence.
ACCESSION   DQ322640
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for 
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology, 
            University of Texas Medical Branch, 301 University Boulevard, 
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 12840)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="VEEV replicon vector YFV-C3opt-NS2mut"
                     /focus
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:364090"
     source          7934..9904
                     /organism="Yellow fever virus 17D"
                     /mol_type="other DNA"
                     /db_xref="taxon:11090"
     misc_feature    2..45
                     /note="5UTR of Venezuelan equine encephalitis virus VEE 
                     TC83"
     misc_feature    46..1650
                     /note="nsP1 of Venezuelan equine encephalitis virus VEE 
                     TC83"
     RBS             1536..1544
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1651..4032
                     /note="nsP2 of Venezuelan equine encephalitis virus VEE 
                     TC83"
     misc_feature    3866
                     /note="A->T mutation from TC83 sequence produce Leu mutant 
                     in nsP2"
     misc_feature    4033..5703
                     /note="nsP3 of Venezuelan equine encephalitis virus VEE 
                     TC83"
     misc_feature    5704..7524
                     /note="nsP4 of Venezuelan equine encephalitis virus VEE 
                     TC83"
     misc_feature    7502..7546
                     /note="subgenomic promoter of Venezuelan equine 
                     encephalitis virus VEE TC83"
     misc_feature    7571..7933
                     /note="Yellow fever virus 17D capsid protein (synthetic, 
                     codon-optimized)"
     misc_feature    7934..8200
                     /label=PR of Yellow fever virus 17D
                     /note="PR of Yellow fever virus 17D"
     misc_feature    8201..8425
                     /label=M of Yellow fever virus 17D
                     /note="M of Yellow fever virus 17D"
     misc_feature    8426..9904
                     /label=E of Yellow fever virus 17D
                     /note="E of Yellow fever virus 17D"
     misc_feature    9910..9953
                     /note="subgenomic promoter of Venezuelan equine 
                     encephalitis virus VEE TC83 (second copy)"
     CDS             9983..10582
                     /codon_start=1
                     /product="puromycin-N-acetyl-transferase"
                     /label=puromycin-N-acetyl-transferase
                     /note="Pac"
                     /protein_id="ABC61539.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    10659..11014
                     /note="3UTR of Venezuelan equine encephalitis virus VEE 
                     TC83"
     promoter        11078..11182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11183..12043
                     /codon_start=1
                     /product="Bla"
                     /function="ampicillin resistance"
                     /label=Bla
                     /protein_id="ABC61538.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12214..12802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        12824..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"