LOCUS       Exported                9637 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YEp24PGK, complete sequence.
ACCESSION   KC562906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Johansson B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2013) Department of Biology, University of Minho, 
            Campus de Gualtar, Braga 4710-051, Portugal
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Cloning vector YEp24PGK"
                     /mol_type="other DNA"
                     /db_xref="taxon:1289963"
     rep_origin      348..1690
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      530..1690
                     /label=2 micron origin
                     /note="2 micron origin"
     protein_bind    680..727
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2291..2506
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2507..3310
                     /codon_start=1
                     /gene="URA3"
                     /product="URA3"
                     /label=URA3
                     /protein_id="AGE61868.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            2507..3310
                     /gene="URA3"
                     /label=URA3
     regulatory      3452..4934
                     /regulatory_class="promoter"
                     /note="from Saccharomyces cerevisiae PGK1"
     regulatory      4941..5305
                     /regulatory_class="terminator"
                     /note="from Saccharomyces cerevisiae PGK1"
     terminator      5030..5300
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     CDS             5363..6553
                     /codon_start=1
                     /gene="tet"
                     /product="tetracyclin resistance protein"
                     /label=tet
                     /note="pBR322 marker"
                     /protein_id="AGE61866.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            5363..6553
                     /gene="tet"
                     /label=tet
     CDS             7194..7385
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    7487..7627
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7813..8401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8572..9432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="AGE61867.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(8572..9432)
                     /gene="bla"
                     /label=bla
     promoter        complement(9433..9537)
                     /gene="bla"
                     /label=AmpR promoter