LOCUS       Exported                3237 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector yeGFP_integration, complete sequence.
ACCESSION   KU904416
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Yu J, Pertusi D, Adeniran A, Tyo KEJ.
  TITLE     CellSort: A support vector machine algorithm for optimizing and 
            simulating FACS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Tyo KEJ., Adeniran A, Stainbrook SC, Bostick JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2016) Chemical and Biological Engineering, 
            Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA
REFERENCE   3  (bases 1 to 3237)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Cloning vector yeGFP_integration"
                     /mol_type="other DNA"
                     /label=derived from Saccharomyces cerevisiae sequence
                     /note="derived from Saccharomyces cerevisiae sequence"
                     /db_xref="taxon:1814215"
     regulatory      1..126
                     /regulatory_class="promoter"
                     /gene="FUS1"
     gene            1..126
                     /gene="FUS1"
                     /label=FUS1
     CDS             130..846
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeast-enhanced green fluorescent protein
                     /note="codons optimized for translation in Saccharomyces 
                     cerevisiae and Candida albicans"
                     /protein_id="AMY56666.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      889..1078
                     /regulatory_class="terminator"
                     /gene="CYC1"
     gene            889..1078
                     /gene="CYC1"
                     /label=CYC1
     terminator      889..1078
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     regulatory      1168..1511
                     /regulatory_class="promoter"
                     /gene="TEF"
                     /note="from Ashbya gossypii"
     gene            1168..1511
                     /gene="TEF"
                     /label=TEF
     promoter        1168..1511
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1512..2087
                     /codon_start=1
                     /gene="NUA4"
                     /product="nourseothricin acetyltransferase"
                     /label=NUA4
                     /note="confers resistance to nourseothricin"
                     /protein_id="AMY56667.1"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     gene            1512..2087
                     /gene="NUA4"
                     /label=NUA4
     regulatory      2095..2282
                     /regulatory_class="terminator"
                     /gene="ADH1"
     gene            2095..2282
                     /gene="ADH1"
                     /label=ADH1
     terminator      2095..2282
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    2410..3237
                     /label=genomic DNA from Saccharomyces cerevisiae ChrIII
                     /note="genomic DNA from Saccharomyces cerevisiae ChrIII"
     CDS             complement(2473..3054)
                     /codon_start=1
                     /gene="HBN1"
                     /product="HBN1"
                     /label=HBN1
                     /protein_id="AMY56668.1"
                     /translation="MSAVATYLKTLTARRTIYALKPELPGEITINDIQSVVQTIIKETP
                     TAFNSQPNRAVILTGETHKKVWDEVTKAIESPAGQKRPASARDEAFGSVIFFTDDKVTE
                     KLKADFPAYAAAFPSFADHTSGAAQINSWVALEAMGLGGHLQHYNGYIKAALPSKIPES
                     WTVQAQLVFGTPAAPPGEKTYIKNDVEIFN"
     gene            complement(2473..3054)
                     /gene="HBN1"
                     /label=HBN1