LOCUS       Exported                5653 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD048, complete sequence.
ACCESSION   KM017899
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Broad host range vector vector pCVD048"
                     /mol_type="other DNA"
                     /note="pANS is a replication device carrying a fragment of 
                     pANS (the small plasmid of Synechococcus elongatus PCC7942)
                     including repA and repB for replication of the plasmid in S
                     elongatus"
                     /db_xref="taxon:1531906"
     misc_feature    29..34
                     /label=ZraI
                     /note="ZraI"
     misc_feature    32..3844
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    32..52
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    53..58
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(257..730)
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /note="repB-pANS; #1 amino acid V to M; similar to INSD 
                     accession AAB21874; Synechococcus elongatus PCC7942"
                     /protein_id="AII99712.1"
                     /translation="MGSCIAPQVRYLHGLKVEELLQRRDWFSVDDPLIQEIVTTATSHR
                     ASAAQILKIQITDKTRASTIVRALLRMIGFKLKARQKRIAGKSTWIYQVVPNEAIAELP
                     LEAIEAHWLENARSSQGLGSCHEIPNRRSKPITAHFRDTASGQVSTTSTETAA"
     CDS             complement(685..3315)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-pANS; similar to INSD accession AAB21875; 
                     Synechococcus elongatus PCC7942"
                     /protein_id="AII99713.1"
                     /translation="MAFIANHLQRSQIPTRCELIDQLDRLPPNWRFCVVNSRKAPFDPG
                     WQNNPLDHAAVAERIRSDRRVTGIGLLTGPASGGLIAVDFDGPTAHEALPEGLTLTELP
                     PTVAYTSGKPGRHQRLYQVPQERWAAIATQKLHSPDGDLLELRWNKLQSVIVGQHPETG
                     AYRWVEGCAPWEIEVAEAPPELLDAMERQDRQTAPKSYKPIVSAPASDDEVAIARTMLA
                     HVPASYADDYESWVAVGMALQSVSDALLDDWIAWSAQSSKFDGNRKLERKWASFKGSGI
                     TIATLAKLAKEGGYRPPKLTRQEQREYQREVANEWQRLTRSPNRLHTGGYINGDYIPQP
                     EQHRLVCLNAAMGTGKTEAIAGHLQPLIGTGIPVVLITHRRTLGAALGKRLGVPWAEEC
                     VPGSDLRMIGLGGCLDSMHPASKLRFSAHGWQRAIVVIDEVELWAEHLLSGKTEIAKHR
                     PEVMAEIAGLLAGGRQVIAADAMLSDVSIALLEALTGSTAYLVSSTVQPFSGAPVYQVG
                     SDRELMAMAVEMVQAGKRLAVFCDRQKLSQSRPSLYSAATIAQQLSEACGQPVPVIDSE
                     ANKRGALETLKLNPQMFINCCPHLVFTSVIDAGLSITQGVGDRPFDAVLIFAGAGHIAP
                     GAIAQIAGRVRSNVPRFITCPDTSNLMRAGNGSIDPTEVADGLKRHQQVILAQLSQAAI
                     KIGEPVASQAYLTAWAELAARRNRDGLTYAATVEQLLRLNGFTWQDWDGAIDPFLQEVV
                     DELQRETAEALADASAEAILSSPDLTPKEAEAIASADFANAEQLAALARHRCQQRWGDC
                     TRELLAADDDGLYAPLRLLFWLSLGKDAVPLAMPPSSTNGAIARCSDPILWAAVSRPKC
                     GTCMG"
     misc_feature    3818..3823
                     /label=SacI
                     /note="SacI"
     misc_feature    3824..3844
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons; synthetic"
     misc_feature    3842..3847
                     /label=ZraI
                     /note="ZraI"
     rep_origin      complement(3870..4543)
                     /direction=LEFT
                     /note="ori-pUC19; origin of replication of pUC19"
     rep_origin      complement(3929..4517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4688..5548)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99711.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5549..5653)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(5549..5618)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"