LOCUS       Exported                4141 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD025, complete sequence.
ACCESSION   KM017913
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Broad host range vector vector pCVD025"
                     /mol_type="other DNA"
                     /note="S7942NS3-RK2BOM is a chromosome integration device 
                     that comprises the recombination site NS3 for Synechococcus
                     elongatus PCC7942, an origin of replication for E coli and 
                     a bom site for conjugal transfer, from pBR322, and a 
                     Tetracycline resistance marker"
                     /db_xref="taxon:1531883"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..2957
                     /note="CVD-BHR-NS; device for the GC-adaptor assembly: BHR 
                     replicon and neutral sites or right arm of a double 
                     recombination KO"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..877
                     /note="S7942NS3-RA; right arm of the homologous 
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     rep_origin      888..1561
                     /note="ori-pBR322; origin of replication of pBR322"
     rep_origin      914..1502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            1685..1988
                     /note="oriT; streamlined origin of transfer from RP4 for 
                     conjugal transfer; PCR amplified from pRL692 INSD accession
                     AF424805"
     oriT            1743..1852
                     /note="incP origin of transfer"
     misc_recomb     2039..2930
                     /note="S7942NS3-LA; left arm of the homologous 
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     misc_feature    2931..2936
                     /label=NheI
                     /note="NheI"
     misc_feature    2937..2957
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2955..2960
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(3081..3941)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99740.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3942..4046)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3942..4011)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    4072..4077
                     /label=ZraI
                     /note="ZraI"