LOCUS       Exported                4098 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD019, complete sequence.
ACCESSION   KM017920
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Broad host range vector vector pCVD019"
                     /mol_type="other DNA"
                     /note="S7942NS1-RK2BOM is a chromosome integration device 
                     that comprises the recombination site NS1 for Synechococcus
                     elongatus PCC7942, an origin of replication for E coli from
                     pBR322 and an oriT site from RK2/RP4 for conjugal transfer"
                     /db_xref="taxon:1531877"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..2914
                     /note="CVD-BHR-NS; device for the GC-adaptor assembly: BHR 
                     replicon and neutral sites or right arm of a double 
                     recombination KO"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous 
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     rep_origin      866..1539
                     /note="ori-pBR322; origin of replication of pBR322"
     rep_origin      892..1480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            1663..1966
                     /note="oriT; streamlined origin of transfer from RP4 for 
                     conjugal transfer; PCR amplified from pRL692 INSD accession
                     AF424805"
     oriT            1721..1830
                     /note="incP origin of transfer"
     misc_recomb     2017..2887
                     /note="S7942NS1-LA; left arm of the homologous 
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     misc_feature    2606..2611
                     /label=XbaI
                     /note="XbaI"
     misc_feature    2793..2798
                     /label=NheI
                     /note="NheI"
     misc_feature    2853..2858
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2888..2893
                     /label=NheI
                     /note="NheI"
     misc_feature    2894..2914
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2912..2917
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(3038..3898)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99750.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3899..4003)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3899..3968)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    4029..4034
                     /label=ZraI
                     /note="ZraI"