LOCUS       Exported               14482 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector Ubi-stop-mCD8GFP, complete sequence.
ACCESSION   KC845568
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14482)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 14482)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Cloning vector Ubi-stop-mCD8GFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1366865"
     regulatory      40..2041
                     /regulatory_class="promoter"
                     /gene="Ubi-p63E"
                     /note="Drosophila melanogaster Ubi-p63E gene promoter"
     gene            40..2041
                     /gene="Ubi-p63E"
                     /label=Ubi-p63E
     misc_recomb     2094..2127
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    2094..2127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2146..4178
                     /regulatory_class="terminator"
                     /note="hsp70Ab transcription terminator"
     polyA_signal    2154..3026
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     misc_recomb     4210..4243
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    4210..4243
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4312..5700
                     /codon_start=1
                     /gene="mCD8-GFP fusion"
                     /product="mCD8-GFP fusion protein"
                     /label=mCD8-GFP fusion
                     /protein_id="AGR55450.1"
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLI?TLICYHSR
                     GSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE?TIFFKDDGNYKTRAEVKFEG
                     DTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGS
                     VQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMD
                     ELYK"
     gene            4312..5700
                     /gene="mCD8-GFP fusion"
                     /label=mCD8-GFP fusion
     CDS             4312..4977
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLI?TLICYHSR
                     "
     CDS             4984..5700
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE?TIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6271..6405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            6757..10302
                     /gene="mini-white"
                     /label=mini-white
     misc_recomb     10455..10778
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    10481..10550
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10904..11489
                     /label=P element 5 end
     rep_origin      complement(11724..12312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12483..13343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AGR55451.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(12483..13343)
                     /gene="bla"
                     /label=bla
     promoter        complement(13344..13448)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    14250..14482
                     /label=P element 3 end
                     /note="P element 3 end"