LOCUS       Exported                9939 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P-element transposon vector UASp, complete sequence.
ACCESSION   AY831681
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Miller DFB., Kaufman TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2004) Biology, Indiana University, 1001 E. Third 
            St., Bloomington, IN 47405, USA
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="P-element transposon vector UASp"
                     /mol_type="other DNA"
                     /note="constructed by P. Rorth from pCasper4 to enhance 
                     Gal4 directed transcription in the germline, Mech. Dev. 78,
                     113-118 (1998)"
                     /db_xref="taxon:304772"
     misc_feature    1..62
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    99..445
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     regulatory      457..701
                     /regulatory_class="promoter"
                     /note="contains P-transposase promoter"
     misc_feature    683..745
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    795..2220
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     mobile_element  complement(2229..2461)
                     /mobile_element_type="transposon:P-element"
                     /note="3 signal sequence"
     misc_feature    2229..2461
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    2965..4993
                     /label=derived from pUC8
                     /note="derived from pUC8"
     promoter        3265..3369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3370..4230
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     mobile_element  complement(3809..5224)
                     /mobile_element_type="transposon:P-element"
                     /note="5 signal sequence"
     rep_origin      4401..4989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5224..5809
                     /label=P element 5 end
     CDS             complement(join(6588..7202,7273..7404,7608..7923,7985..8639,
                     8714..8987,9389..9460))
                     /codon_start=1
                     /product="white"
                     /label=white
                     /protein_id="AAV97853.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"